Interdependence investigation of dynamic between the von Willebrand factor and erythrocyte and leukocyte intoxication indices in experimental peritonitis

I. V. Savytskyi, S. V. Tsypoviaz, R. G. Lenik, O. V. Bilash, R. H. Zashchuk, I. V. Miastkivska, L. M. Zaiats

Abstract


The research was conducted on 175 white rats of reproductive age (3 months), weight of animals - 180-220 g. Animals were divided into 4 groups:
Group 1 - 20 intact animals.
Group 2 - 50 rats with simulated fecal peritonitis.
Group 3 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction and debridement by chlorhexidine solution.
Group 4 - 50 rats with simulated fecal peritonitis with subsequent antibiotic correction, chlorhexidine debridement and endothelial dysfunction correction with the use of a nitric oxide donor.
Fecal peritonitis was modeled using injection of 10% fecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method (Lazarenko V.A., et al., 2016, patent No. 233826).
The following results were obtained.
Endothelial dysfunction is the trigger mechanism of vascular catastrophes after experiencing experimental peritonitis.
Evidence of endothelial functional status impairment during experimental peritonitis is a significant increase in the level of Willebrand factor in the animals blood flow (p <0.001).
It was revealed significant increase in erythrocyte intoxication index (EII).
It has been proved increase in leukocyte intoxication index (LII) in experimental animals during simulated peritonitis.
It has been confirmed effectiveness of nitric oxide donor use in the complex correction of peritonitis and endothelial dysfunction as its complication is confirmed.

Keywords


peritonitis; experimental research; von Willebrand factor; erythrocyte intoxication index (EII); leukocyte intoxication index (LII); L-arginin

Full Text:

PDF

References


Kumar S, Kumar S, Kumar S, Gautam S. Spontaneous gallbladder perforation in a patient of situs inversus totalis, misdiagnosed as perforation peritonitis due to gas under the right dome of the diaphragm. BMJ Case Rep [Internet]. 2015 Jun [cited 2017 Sep 22]; 2015:1-3. Available from: https://www.ncbi.nlm.nih.gov/labs/articles/26123454/ doi: 10.1136/bcr-2014-208003

Riché FC, Dray X, Laisné MJ, Matéo J, Raskine L, Sanson-Le Pors MJ, et al. Factors associated with septic shock and mortality in generalized peritonitis: comparison between community-acquired and postoperative peritonitis. Critical Care [Internet]. 2009 [cited 2017 Sep22];13(3):R99. Available from: https://www.ncbi.nlm.nih.gov/pubmed/19552799 doi:10.1186/cc7931

Kim T, Hong SI, Park SY, Jung J, Chong YP, Kim SH, et al. Clinical Features and Outcomes of Spontaneous Bacterial Peritonitis Caused by Streptococcus pneumoniae: A Matched Case-Control Study. Medicine (Baltimore) [Internet]. 2016 May [cited 2017 Sep 22]; 95(22): e3796. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900721/ doi: 10.1097/ MD.0000000000003796

Bektas H, Kleine M, Tamac A, Klempnauer J, Schrem H. Clinical Application of the Hanover Classification for Iatrogenic Bile Duct Lesions. HPB Surg [Internet]. 2011 [cited 2017 Sep 22]; 2011:10p. Available from: https://www.hindawi.com/journals/hpb/2011/612384/ doi: 10.1155/2011/612384

Mazuski JE, Solomkin JS. Intra-abdominal infections. Surg. Clin. N. Am. 2009;89;2:421-437.

Sartelli M. A focus on intra-abdominal infections. World J. Emerg Surg. 2010;19:5-9.

Kupreeva MS, Petrosjan JeA, Suhinin AA i dr. Ocenka sostojanija krasnoj krovi pri zhelchnom peritonite. Bjulleten' Volgogradskogo nauchnogo centra RAMN. 2008;2:49-51.

Savel'ev VS, Petuhov VA, An ES, Semenov ZhS, Mironov AV. Disfunkcija jendotelija pri lipidnom distress–sindrome i dismetabolicheskih posledstvijah peritonita. RMZh. 2009;14:881

Lazarenko V.A., Lipatov V.A., Blinkov Ju.Ju., Skorikov D.V. Jeksperimental'naja model' rasprostranennogo kalovogo peritonita. Kurskij nauchno-prakticheskij vestnik "Chelovek i ego zdorov'e", 2008, № 4.S.128-132.

Pokrovskij M.V., Pokrovskaja T.G., Korchakov V.I. i dr. Jendotelioprotektornye jeffekty L-arginina pri modelirovanii deficita okisi azota. Jeksperim. i klin. farmakol. 2008; 71 (2): 29–31.

Dinamіka pokaznikіv endogennoї іntoksikacії u shhurіv rіznogo vіku za umov gostrogo otruєnnja toksinami blіdoї poganki / І. P. Kuz'mak, І. M. Klіshh, O. Z. Jaremchuk // Naukovij vіsnik Uzhgorods'kogo unіversitetu. Serіja : Bіologіja. – 2012. – Vip. 33. – S. 154–155.

Kal'f-Kalif Ja. Ja. O lejkocitarnom indekse intoksikacii i ego prakticheskom znachenii // Vrachebnoe delo.— 1941.— № 1.— S. 31–33.

Harlamova U.V.,Il'icheva O. E. Diagnosticheskoe znachenie laboratornyh markerov povrezhdenija jendotelija u bol'nyh na programmnom gemodialize// Klinicheskaja laboratornaja diagnostika.- 2012.-№ 7.-S.39-41.

Barkagan Z.S., Momot A.P. Diagnostika i kontroliruemaja terapіja narushenij gemostaza.. M. Izd. «N'judiamed». 2001. 296 s.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2020 I. V. Savytskyi, S. V. Tsypoviaz, R. G. Lenik, O. V. Bilash, R. H. Zashchuk, I. V. Miastkivska, L. M. Zaiats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

Journal Language(s): English 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2  www.ukw.edu.pl Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy http://ojs.ukw.edu.pl/index.php/johs  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase Index.com.

Website: www.infobaseindex.com

Universal Impact Factor 1.78 for year 2012. (http://www.uifactor.org/AppliedJournals.aspx)

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)