Arterial hypertension as a predictor of morpho- densitometric changes development in rats` solitary-vagal complex

M. V. Danukalo, O. V. Melnikova

Abstract


The components of the dorsal vagal complex: the nucleus of the solitary tract (NST) and the dorsal motor nucleus (DMN) are key structures in BP regulation. The maintenance of BP normal level depends on functional state of these structures. it is possible to estimate the neuron morpho-functional state not only with direct electrophysiological methods but also analyzing the nucleus size and nucleic acid (NA) concentration in it. Therefore, the aim of our work was to determine the features of morpho-densitometric indices in the nuclei of the NST and DMN neurons in AH of various origins in experimental animals and to give a comparative analysis of these data. Materials and methods: the study was carried out on 30 mature male rats: 10 control Wistar rats, 10 Wistar rats with modeled endocrine-salt AH (ESAH) and 10 spontaneously hypertensive rats (SHR) which are well-established model of human essential arterial hypertension (EAH). The histochemical method of staining with gallocyanine-chrome alum by Einarson was used for the evaluation of morpho-densitometric characteristics of the DMN and NTS neurons` nuclei: the nucleus area, the content and concentration of nucleic acids in the nucleus.
Conclusions: The following conclusions can be made as a research result: 1. Morpho-densitometric changes occur in NTS and DMN in arterial hypertension, regardless of its pathogenesis.
2. The most distinct changes of the studied indices of neurons` nuclei were observed in DMN of the rats with EAH. Although only the neurons` nuclei area significantly changed in the NTS. The distribution of neurons` nuclei by their area in DMN and NTS in SHR rats showed a downward trend.
3. Significant decrease in all morpho-densitometric characteristics of the neurons` nuclei is observed in the DMN in the rats with an ESAH. The distribution of neurons` nuclei by their area showed a tendency to decrease. In the nuclei of NST neurons, only the content and concentration of NA were reliably low in comparison with control animals. The distribution curve of neurons by the area of their nuclei in ESAH rats is characterized by wider variability of size than in the control group.

Keywords


nucleus of the solitary tract, dorsal motor nucleus, arterial hypertension, rats

Full Text:

PDF

References


Marina, N., Ang, R., Machhada, A., Kasymov, V., Karagiannis, A., Hosford, P. S & Kasparov, S. (2015). Brainstem hypoxia contributes to the development of hypertension in the spontaneously hypertensive rat. Hypertension, 65(4), 775-783.

Kolpakova, J., Li, L., Hatcher, J. T., Gu, H., Zhang, X., Chen, J., & Cheng, Z. J. (2017). Responses of Nucleus Tractus Solitarius (NTS) early and late neurons to blood pressure changes in anesthetized F344 rats. PloS one, 12(4), e0169529.

Hosford, P. S., Millar, J., Ramage, A. G., & Marina, N. (2017). Abnormal oxygen homeostasis in the nucleus tractus solitarii of the spontaneously hypertensive rat. Experimental physiology, 102(4), 389-396.

Gregory, M. D., & Benarroch, E. E. (2017). Nucleus of the solitary tract, medullary reflexes, and clinical implications.

Sabbatini, M., Grossini, E., Molinari, C., Mary, D. A., Vacca, G., & Cannas, M. (2017). Gastric distension causes changes in heart rate and arterial blood pressure by affecting the crosstalk between vagal and splanchnic systems in anesthetised rats. Experimental brain research, 235(4), 1081-1095.

Gourine, A. V., Machhada, A., Trapp, S., & Spyer, K. M. (2016). Cardiac vagal preganglionic neurones: An update. Autonomic Neuroscience, 199, 24-28.

Cheng, Z., Zhang, H., Guo, S. Z., Wurster, R., & Gozal, D. (2004). Differential control over postganglionic neurons in rat cardiac ganglia by NA and DmnX neurons: anatomical evidence. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 286(4), R625-R633.

Blanch, G. T., Freiria-Oliveira, A. H., Speretta, G. F. F., Carrera, E. J., Li, H., Speth, R. C., ... & Colombari, D. S. (2014). Increased expression of angiotensin II type 2 receptors in the solitary–vagal complex blunts renovascular hypertension. Hypertension, 64(4), 777-783.

Dornas, W. C., & Silva, M. E. (2011). Animal models for the study of arterial hypertension. Journal of biosciences, 36(4), 731-737.

Kolesnyk Y, Gancheva O, Abramov A, Ivanenko T, Tischenko S, Kuzo N, inventors; Zaporizhzhya State Medical University, assignee. Sposib modelyuvannya symptomatychnoy arterial`noi hypertezii u dribnyh gryzuniv [Method of simulation of symptomatic arterial hypertension in small rodents]. Ukraine patent 102234, 2015 Oct. 26. (Ukrainian)

Kolesnyk, Yu. M., Hancheva, O. V., Abramov, A. V., Kolesnyk, M. Yu., Ivanenko, T. V., Tishсhenko, S. V., et al. (2017). Sovremennye podkhody i novye metodicheskie vozmozhnosti v ocenke funkcional’nogo sostoyaniya melkikh laboratornykh zhivotnykh [Modern approaches and new methodological possibilities in the functional state of small laboratory animals assessing]. Pathologia, 3(41), 364–370. doi: 10.14739/2310- 1237.2017.3.118770 [in Russian]

Paxinos G, Watson C. The Rat in Stereotaxic Coordinates. New York, Academic Press; 1986.

Pearse, A. G. E. (1962). Gistokhimiya. Teoreticheskaya i prikladnaya [Histochemistry, Theoretical and Applied]. Moscow: Izdatel`stvo inostrannoy literatyri. [in Russian].

Ferreira, T., & Rasband, W. (2012). ImageJ user guide. ImageJ/Fiji, 1, 155-161.

Zaycev V, Liflyandskiy V. Prikladnaya meditsynskaya statistika. Uchebno-practicheskoye posobiye. [Applied medical statistics. Teaching aid] 1st ed. Sankt-Peterburg: Foliant; 2003. [in Russian]

Lafarga, M., & Berciano, M. T. (1985). A Golgi and morphometric study of the ectopic granule cells in the molecular layer of the rat cerebellum. Brain research, 345(2), 398-401.

Nelson, D. O., & Boulant, J. A. (1983). Altered brainstem structure of spontaneously hypertensive (SHR) rats. Brain research, 261(1), 145-150.

Basting, T., & Lazartigues, E. (2017). DOCA-salt hypertension: an update. Current hypertension reports, 19(4), 32.

McMenamin, C. A., Anselmi, L., Travagli, R. A., & Browning, K. N. (2016). Developmental regulation of inhibitory synaptic currents in the dorsal motor nucleus of the vagus in the rat. Journal of neurophysiology, 116(4), 1705-1714.

Melo, M. R., Gasparini, S., Speretta, G. F., Silva, E. F., Pedrino, G. R., Menani, J. V., Colombari, E. (2019). Importance of the commissural nucleus of the solitary tract in renovascular hypertension. Hypertension Research, 42(5), 587.

Tsai, H. W., & Davenport, P. W. (2016). Activation of Neurons in the Intermediate and Medial Nucleus of Tractus Solitarius by Multiple Afferent Stimuli. The FASEB Journal, 30(1_supplement), 1261-6.




DOI: http://dx.doi.org/10.5281/zenodo.3497436

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 © The Author (s) 2019

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

Journal Language(s): English 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2  www.ukw.edu.pl Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy http://ojs.ukw.edu.pl/index.php/johs  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 2011: 5.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase Index.com.

Website: www.infobaseindex.com

Universal Impact Factor 1.78 for year 2012. (http://www.uifactor.org/AppliedJournals.aspx)

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)