Evaluation of the influence of Chloride Sodium rape on the external application on the structural and functional condition of rats kidney on development

S. Gushcha, B. Nasibullin, W. Zukow, I. Savitskyi, V. Volyanska


       Much attention has been paid to the study of the numerous effects of chronic stress and the methods of their correction, but some questions remain unanswered. Goal. To investigate the effect of highly mineralized sodium chloride mineral water (brine) on the structural and functional state of rat kidneys with experimental distress. White female rats weighing 180 - 220 g were ranked into three groups: group I intact rats (control), group II rats with a distress model (duration 30 days); group III - rats with a model of distress, who from 15 to 30 day of experiment received a course of external procedures with rapes. In group II rats, the development of distress caused dystrophic changes in the kidneys, which led to inhibition of their urinary, ion-regulating and excretory functions. To correct the manifestations of distress rats received a course of external procedures with brine (5 procedures for 2 hours a day). Renal function restored: GFR exceeds group I data by 30%, resulting in complete recovery of the diuresis. Urea excretion is restored, and creatinine excretion exceeds the control level by 30% (p <0.001). The concentration of potassium ions is restored to the control level, their excretion is reduced by 20%, and the urinary excretion of sodium and chloride ions is increased by — 90% and 57%. Microscopically — the structure of nephrons and their components without visual changes, that is, does not differ from the first group of rats. Conclusions. Studies have shown that with transdermal administration of sodium chloride brine has a significant protective effect on the course of pathological changes in the function of the kidney of rats against the background of the development of distress.


distress; functional state of kidneys; brine.

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Mineral waters of Ukraine / Ed. EO. Kolesnika, KD. Babov. – Kyiv: Kupriyanov, 2005: 576 p.

Quattrini S, Pampaloni В, Brandi ML. Natural mineral waters: chemical characteristics and health effects. Clin Cases Miner Bone Metab. 2016 Sep-Dec; 13(3): 173–180. doi: 10.11138/ccmbm/2016.13.3.173.

Physiology: national leadership / under. ed. GN. Ponomarenko. – Moscow: GEOTAR-Media, 2009: 864 p.

Zolotareva TA, Babov KD, Nasibullin BA. et al. Medical rehabilitation. – Kyiv: KIM, 2012: 496 p.

Vladimirsky EV, Filtsagina TN. Problems of antihypertensive balneotherapy. Problems of balneology, physiotherapy and exercise therapy. 2013; 5: 40–45.

Medical-hydrogeochemical factors of the geological environment of Ukraine (Ed By HI. Rudko. Kyiv – Chernovtsy: Burek, 2015: 724 p.

Joel E, Dimsdale MD. Psychological Stress and Cardiovascular Disease. J Am Coll Cardiol. 2008 Apr 1; 51(13): 1237–1246. doi: 10.1016/j.jacc.2007.12.024.8. 8. Mariotti А. The effects of chronic stress on health: new insights into the molecular mechanisms of brain–body communication. Future Sci OA. 2015 Nov; 1(3): FSO23. doi: 10.4155/fso.15.21.

Pshennikova M. G. The phenomenon of stress. Emotional stress and its role in pathology // Pathological physiology and experimental therapy. 2001; 4: 28–40.

Pervanidou P, Chrousos GP. Metabolic consequences of stress during childhood and adolescence. Metabolism. 2012; 61(5): 611–619. doi: 10.1016/j.metabol.2011.10.005.

Bakholdina EI, Zolotareva TA, Oleshko AYa. A new method of biotesting manifestations of endogenous intoxication. Word of Medicine and Biology. 2013; 4: 63–65.

Alekseenko NA, Gushcha SG, Ivanova AV, Bondar IV. Modeling the state of chronic stress to study the stress-protective properties of natural healing resources. Medical rehabilitation, balneology, physical therapy. 2010; 3 (63): 17–20.

Rougier A. Percutaneous absorbtion-transepidermal water loss relationship in man in vivo / А. Rougier іn R. Scott, R.H. Guy, J. Hadgraft (eds.). Prediction of Percutaneous Penetration JBC Technical services, London. 1991; 2: 60–72.

Gushcha SG, Bondar YuP, Babova IK, Balashova IV, Plakida AL. Іnvestigation of the effect of natural chloride-magnesium solution on the functional state of kidneys with experimental arthrosis. Deutscher Wissenschaftsherold German Science Herald. 2017; 5: 16–20.

Schmook FP, Andreas В, Meingassner JG. Comparison of human skin or epidermis models with human and animal skin in in-vitro percutaneous absorption. International Journal of Pharmaceutics. 2001; 215 (1-2): 51–56. DOI: 10.1016/S0378-5173(00)00665-7.

Schaefer H, Redelmeier TE. Structure and dynamics of the skin barrier. Skin barrier. — Tokyo, Japan: Karger, 1991: 1–33.

Lin TK,  Zhong L,  Santiago JL. Anti-Inflammatory and Skin Barrier Repair Effects of Topical Application of Some Plant Oils. Int J Mol Sci. 2018 Jan; 19 (1): 70. doi: 10.3390/ijms19010070.

Ulashchik VS. Skin receptors and therapeutic physical factors. Problems of balneology, physiotherapy and exercise therapy. 2017; 94: 2: 48–57.

Instruction 2010/63/EU of European Parliament and Council on animals used for research and other purposes protection. Official Journal. 2010;276:33–79.

Pat. 61221 Ukraine, IPC G09B 23/28. A method of modeling chronic stress. TA Zolotaryova, BA Nasibullin, NO Alekseyenko, SG Gusta, GV Ivanova, IV Bondar. – No u 2010 15935; declc. 12/29/2010; publ. 11.07.2011. Bul. № 13.

Order of MOH of Ukraine No. 692 dated 28.09.2009 «On approval of the recommendations of the research methods of biological effects of natural medicinal resources and preformed remedies».

Mineral healing waters. Specifications. GSTU 42.10-02-96. Kyiv: Ministry of Health.1996: 30 p.

DOI: http://dx.doi.org/10.5281/zenodo.3491052


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