Morphological changes in kidney glomeruli of rats with chronic toxic hepatitis and its correction with lisinopril, L-arginine-L-glutamate and their combination

N. A. Rykalo, Yu. M. Berehovenko, O. V. Androshchuk


The article presents structural changes of glomerular component of kidneys in rats with chronic toxic hepatitis (CTH) and the results of its correction with lisinopril and L-arginine L-glutamate. The influence of ACE inhibitors or angiotensin-blockers (AT) II on RAS chains is known to result in positive therapeutic effect. Addition of lisinopril to complex therapy has positively affected not only the clinical manifestations of the disease but also the functional status of kidneys, primarily due to the influence on filtration processes.
Considering the significance of RAS activation in the development of hepatorenal syndrome, the aim of the work was to study the influence of ACE inhibitors (lisinopril) on kidney morphological structure during modeling of CTH, to compare its effect with hepatoprotector L-Glutargin-L-glutamate as well as to evaluate structural changes in kidney glomeruli after combined use of those drugs. The experimental study was conducted on 60 white laboratory immature rats, with initial body weight of 50-70g. CTH was modeled by intragastric administration of CCl4 oil solution in a dose of 0.1 ml / 100 g of the body weight twice a week for eight weeks. Drug correction was performed by introduction of lisinopril (in a dose of 20 mg / kg) and L-arginine-L-glutamate (in a dose of 30 mg / kg).
The study revealed pronounced morphological changes in kidney glomeruli (destruction of glomerular vessels and cells) in CTH. The use of corrective factors in the experiment made it possible to decrease the severity of pathological changes. The use of lisinopril resulted in restoration of glomerular structure. After administration of L-arginine-L-glutamate, decrease of degenerative changes in kidney glomeruli was detected. Combined use of both chemical compounds mutually potentiated their action and was accompanied by membrane-stabilizing effect and intensification of aforementioned restoration processes in structural components of nephron glomeruli. Thus, it was established that after 90 days of experimentally modeled CTH, pronounced morphological changes in kidney glomeruli (destruction of vessels and glomerular cells) occurred.


chronic toxic hepatitis; rats; lisinopril; L-arginine-L-glutamate; morphological changes in kidney glomeruli

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Babak O.Ia. Fybroz pecheny: sovremennie predstavlenyia o mekhanyzmakh, sposobakh dyahnostyky y lechenyia [Liver fibrosis: current understanding of the mechanisms, methods of diagnosis and treatment]. Suchasna hastroenterolohiia;2009, №2: 5-17. (in Ukrainian)

Kovalenko V.M., Lutai M.I., Sirenko Yu.M., Sertsevo-sudinni zahvoryuvannya. Klasifikatsiya, standarti diagnostiki ta likuvannya [Cardiovascular disease: сlassification, standards of diagnosis and treatment]. Scientific and production publication; KiYiv, 2007; 128. (in Ukrainian)

Kvasnytska O.B, Hozhenko A.I. Mozhlyvosti farmakolohichnoi korektsii renalnoi dysfunktsii u khvorykh na dekompensovanyi tsyroz pechinky [Possibilities of pharmacological correction of renal dysfunction in patients with decompensated cirrhosis of the liver]. Aktualnie problemi transportnoi medytsyni; 2016, № 2 (44). (in Ukrainian)

Kvasnytska O.B., Hozhenko A.I. Stan funktsionalnoho nyrkovoho rezervu, yak rannii diahnostychnyi kryterii hepatorenalnoho syndromu u khvorykh na tsyroz pechinky [Functional renal reserve state as an early diagnostic criterion for hepatotoxic syndrome in patients with cirrhosis of the liver]. Aktualnie problemi transportnoi medytsyni; 2015, №3, t. 2 (41II). (in Ukrainian)

Nahymullyn R.R., Ustymov D.Iu., Davidova V.R., Shypulyn F.A. Dyahnostycheskye kryteryy hepatorenalnoho syndroma y sovremennie metodi terapyy [Diagnostic criteria of hepatorenal syndrome and modern methods of therapy]. «Ranуу y rannevie ynfektsyy» zhurnal ym. prof. B.M. Kostiuchenka, 2017; T.4(2): 6-11. (in Russian)

Pat. 43704 Ukrainy (Pat. 43704 Ukrainy, MPK (2009) G09V 23/00. Sposib modeliuvannia khronichnoho toksychnoho hepatytu ta tsyrozu pechinky u nestatevozrilykh shchuriv [Method for simulating chronic toxic hepatitis and liver cirrhosis in non-pregnant rats]. vlasnyk Vinnytskyi natsionalnyi medychnyi universytet im. M. I. Pyrohova; № u2009 03490; zaiavl. 10.04.2009 ; opubl. 25.08.2009, Biul. №16. (Pat. 43704 Ukrainy, MPK (2009) G09V 23/00. (in Ukrainian)

Rudemiller N.P., Pater M.B. C-C Motif Chemokine 5 Attenuates Angiotensin II-Dependent kidney injury by limiting renal macrophage infiltration. American journal of pathology; 2016, №186 (11): 2846-2856. (in USA)

Ruilope L.M. Renin-angiotensin-aldosterone system blockade and renal protection: angiotensin/converting enzyme inhibitors or angiotensin II receptor blockers. Acta Diabetol; 2005, Vol.42.: 33-41. (in Italia)

Rykalo N.A., Berehovenko Yu.M. Strukturni zminy epitelialnoho ta klubochkovoho komponentiv nyrok shchuriv pry modeliuvanni khronichnoho toksychnoho hepatytu ta korektsii lizynoprylom [Structural changes of the epithelial and glomerular components of kidney of rats in the modeling of chronic toxic hepatitis and correction of lisinopril]. Aktualni problemy transportnoi medytsyny; Odesa, 2017, № 2 (48): 104-109. (in Ukrainian)

Sorochynnykov A. P. Hystolohycheskaia y mykroskopycheskaia tekhnyka: rukovodstvo [Histological and microscopic technique: a guide]. Smolensk : "SAU"; 2000, 476. (in Russian)

Stefanov O. V. Doklinichni doslidzhennia likarskykh zasobiv (metodychni rekomendatsii). K. [ Preclinical research of medicinal products (methodical recommendations)]. MOZ Ukrainy, Derzhavnyi farmakolohichnyi tsentr. 2001, 527. (in Ukrainian)

Yim H.E., Yoo K.H. Early Treatment with enalapril and leter renal injury in programmed obese adult rats. Journal of cellular physiology; 2017, №232 (2): 447-455. (in Korea)



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