Influence of oral fatty applications on biochemical indicators of inflammation and dysbiosis in the tissues of the rat mouth

A. V. Markov, Iu. Z. Labush, I. V. Khodakov, A. P. Levitsky, G. N. Varava


Background. The negative impact on the body of high-fat nutrition largely depends on the fatty acid composition of edible fats and the presence of peroxidation products in them, which are formed during heat treatment. Despite the considerable number of studies on this issue, it remains unclear the nature of the influence of different dietary fats on the condition of the tissues of the oral cavity. The purpose of this study was to clarify this issue
Methods. Ordinary (high-linoleic) sunflower oil, high-oleic sunflower oil and butter in the native state and after heat treatment were used. The experiments were carried out on 35 white rats with an average weight of 252±15 g, who were given oral applications of the above fats at a dose of 0.5 ml per rat for 3 days. After rat killing on the 4th day, the content of malondialdehyde (MDA), the activity of elastase, urease, lysozyme and catalase were determined in the cheek mucosa homogenate and in the gums. The degree of dysbiosis was calculated by the ratio of the relative activities of urease and lysozyme. The antioxidant-prooxidant API index was calculated from the ratio of catalase activity and MDA content
Results. Oral applications of native and heat-treated oils cause a decrease in the MDA content in both tissues with the exception of heat-treated butter, applications of which do not reduce the MDA level.
Applications of heat-treated oils increase the activity of elastase in the gums, and heat-treated sunflower oil in the cheek mucosa.
Applications of native and heat-treated oils reduce the activity of catalase in the cheek and gums. The API index increases in the gums under the action of native oils and decreases under the action of thermally processed oils.
Applications of butter (native and heat-treated) significantly reduce the activity of urease in the cheek mucosa. Sunflower and high-oleic sunflower oils tend to increase the activity of urease, the least pronounced for the latter.
All oils except high-oleic sunflower, reduce the activity of lysozyme in both tissues and all oils increase the degree of dysbiosis (except butter in the cheek mucosa).
The content of diene conjugates after heat treatment increased the most in butter, and the least in high-oleic sunflower oil.
Conclusion. Oral applications of fats cause a decrease in the formation of active forms of oxygen in the tissues of the oral cavity, which causes the development of dysbiosis and inflammatory processes.


fats, oral cavity, dysbiosis, lipid peroxidation

Full Text:



Lemoine R. Une guerre pour rien? Rev. lait. fr. 2002; 620: 45.

Levitsky AP. The ideal formula of fatty food. Odessa, KP of the OSG, 2002: 61. (in Russian)

Novgorodtseva TP, Karaman IuK, Zhukova NV. Modification of the fatty acid composition of polar and neutral blood lipids and rat liver tissue in conditions of prolonged high-fat diet. Biomedical chemistry. 2013; 59(6): 644-654. (in Russian)

Ivashkin VT, Maevskaya MV. Lipotoxicity and metabolic disorders in obesity Russian Journal of Gastroenterology, Gepatology, Coloproctology. 2010; 20(1): 4-13. (in Russian)

Gozhenko AI, Levchenko EM, Levitsky AP. The hepatoprotective effect of quertulin in rats with disbiosis after high-fat diet. Journal of Health Sciences. 2013; 3(9):339-346.

Levitsky Anatoly, Maykova Anna, Makarenko Olga. Some indicators in rat blood samples taken from the portal vein and the inferior vena cava after consumption of different edible fats. Journal of Education, Health and Sport. 2018; 8(5): 299-308.

Unger RH. Lipotoxic Diseases. Annu. Rev. Med. 2002; 53: 319-336.

Levitsky AP, Khodakov IV, Levchenko EM. Influence of high fat diets with different composition of fatty acids on the content of essential fatty acids in liver lipids.Journal of Education, Health and Sport. 2015;5(12):598-607.

Chertkov NI, Lugovoi AV, Sergeev AG, Mironova AN. Storage of vegetable oils and fats. M., Agropromizdat, 1989: 288. (in Russian)

Khajuria A. Lipid peroxidation. Everyman’s Sci. 1997; 32(3): 109-113.

Narasinhamurthy K, Raina PL. Long term feeding effect of thermally oxidosed oils on antioxidant enzymes in rats. Indian J. Exp. Biol. 1999; 37(10): 1042-1045.

Levitsky AP. Olivka: the unique sunflower oil, the analogue to olive oil. Odessa, KP of the OSG, 2016: 27. (in Russian)

Levitsky AP, Denga OV, Makarenko OA [and others]. Biochemical markers of inflammation of the tissues of the oral cavity: methodical recommendations. Odessa, KP of the OSG, 2010: 16. (in Russian)

Levitsky AP, Makarenko OA, Selivanskaya IA [and others]. Enzymatic method for determining oral dysbiosis for screening pro and prebiotics: guidelines. Kiev, State Pharmacological Center, 2007: 22. (in Russian)

Levitsky AP. Lysozyme instead of antibiotics. Odessa, KP of the OSG, 2005: 74. (in Russian)

Gavrilov BV, Gavrilova AR, Khmara NF. Measurement of diene conjugates in plasma by UV absorption of heptane and propanol extracts. Laboratory work. 1988; 2: 60-63. (in Russian)

Levitsky AP, Makarenko OA, Khodakov І. V. Methods to investigate fats and oils. Odessa, KP of the OSG, 2015: 32. (in Russian)

Skulachev VP. The possible role of reactive oxygen species in protecting against viral infections. Biochemistry. 1998; 63(12): 1691-1694. (in Russian)



  • There are currently no refbacks.

Copyright (c) 2018 © The Author (s) 2018

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:

Journal Language(s): English 

PBN Poland



Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2 Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase


Universal Impact Factor 1.78 for year 2012. (

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski