The level of total bile acids in bile decreased in the groups of experimental animals in comparison with the control group. In the group of animals with simulated ischemic-reperfusion injury level of total bile acids in bile reached the minimal values in on the 3rd day, increasing was showed after on the 7th day, however level still remained lower than in control group. In EG-2 and EG-3 there was a unidirectional decreasing of the index by the seventh day, but in EG-3 these changes were more significant. Consequently, the level of cholesterol in the bile due to simulated injuries decreased in EG-1 and reached the minimal values in on the 3rd day and slightly increased to the seventh day of observation. In EG-2 the index gradually increased to the seventh day of observation. In EG-3 the maximum increasing was observed until the third day, after which it was at the same level. The analysis of the obtained indices testifies to the negative influence of ischemic-reperfusion syndrome on the level of cholesterol in the bile. Described changes influenced the cholato-cholesterol ratio, which was decreasing in all experimental groups. The most significant changes were in EG-3 in which abdominal trauma and hypovolemic shock were combined with ischemic-reperfusion limb syndrome.
In the conditions of an experimental trauma with an ischemic-reperfusion injury of the lower limbs, there is a violation of the indices of the biliary function of the liver, which manifest themselves as a significant reduction of the level of total bile acids in the bile with increasing cholesterol concentration.

biliary production, trauma, bleeding, reperfusion syndrome, experiment.

Elias-Miró M, Jiménez Castro M, Peralta C. (2012) Ischemia-Reperfusion Injury Associated with Liver Transplantation in 2011: Past and Future.

Zhai Y, Petrowsky H, Hong JC, Busuttil RW, Kupiec-Weglinski JW. (2013) Ischaemia-reperfusion injury in liver transplantation-from bench to bedside. Nat Rev Gastroenterol Hepatol., 10(2), 79-89.

Eltzschig HK, Collard CD. (2004). Vascular ischaemia and reperfusion injury. Br. Med. Bull.,70, 71-86.

Videla L.A. (2009) Oxidative stress signaling underlying liver disease and hepatoprotective mechanisms. World J Hepatol., 1(1), 72-8.

Dimitrios Papadopoulos, Thomas Siempis, Eleni Theodorakou, Georgios Tsoulfas (2013). Hepatic Ischemia and Reperfusion Injury and Trauma: Current Concepts . Archives of Trauma Research., 2(2), 63-70.

Peter Fickert , Martin Wagner (2017). Biliary bile acids in hepatobiliary injury – What is the link? Journal of Hepatology., 67, 619–631.

Valeria Mariotti, Mario Strazzabosco, Luca Fabris, Diego F. Calvisi (2018). Animal models of biliary injury and altered bile acid metabolism. Biochimica et Biophysica Acta, 1864 (4), 1254-1261.

A.A. Hudyma, V.V. Yarema (2012). Dynamika morfometrychnykh pokaznykiv ta yikh korelyatsiya z letalʹnistyu u tvaryn iz riznoyu metabolizuvalʹnoyu zdatnistyu pechinky v rannʹomu periodi politravmy [Dynamics of morphometric indices and their correlation with mortality in animals with different metabolic capacity of the liver in the early period of the polytrauma]. Shpytalʹna khirurhiya, 4 (60), 65–67. [in Ukraine]

A.A. Hudyma, V.V. Yarema (2012). Porushennya zhovchoutvorennya i zhovchovydilennya v ranniy period politravmy u tvaryn z riznoyu metabolizuvalʹnoyu zdatnistyu pechinky [Violation of bile formation and bile excretion in the early period of polytrauma in animals with different metabolic capacity of the liver]. Zdobutky klinichnoyi i eksperymentalʹnoyi medytsyny. 2 (17), 48–52. [in Ukraine]

T.A. Zayetsʹ, A.A. Hudyma (2014). Stan zhovchovydilʹnoyi funktsiyi pechinky v umovakh kranioskeletnoyi travmy, uskladnenoyi krovovtratoyu [Bile-excretory function of the liver in conditions of cranioscheletal trauma complicated by blood loss]. Shpytalʹna khirurhiya, 2, 38–41. [in Ukraine]

F.M. Sokolova, T.G. Toporuk, V.P. Bersnev (2005). Adaptivnyye vozmozhnosti ranney reabilitatsii u detey s tyazheloy CHMT [Adaptive possibilities of early rehabilitation in children with severe TBI]. Aktual'nyye voprosy nevrologii i neyrokhirurgii: sb. nauch. tr. – Rostov-na-Donu. 112–113. [in Russian]