Condition of the liver’s liver of rats, received a high-fat diet: biochemical indicators

V. L. Vasyuk, A. P. Levitsky

Abstract


Aim: To determine the liver condition of rats fed with fats of different fatty acid compositions Materials and methods: We used the following dietary fats: refined sunflower oil, refined olive oil, palm oil, milk fat (butter). Feeding of rats was carried out for 41 days using 15 % of the studied oils in the food. The liver condition was evaluated by biochemical markers of liver (elastase, MDA, alkaline phosphatase (ALP), lysozyme, catalase) and blood serum markers (glucose, cholesterol, elastase, ALT, ALP). Results: The increase in the weight gain was more pronounced when feeding with palm oil; the increase of inflammatory markers levels (MDA and elastase) was greater when feeding with palm oil and butter. Conversely, the lysozyme activity in the liver was significantly reduced at fat feeding, particularly for palm oil and butter. In the serum of all animals fed with fat there was the significant increase of glucose and cholesterol levels, as well as the increase of the elastase activity (after palm oil). Conclusion: A high-fat diet causes the development of an initial phase of inflammatory and degenerative processes in liver, which is more pronounced for fats high in palmitinic acid (palm oil and butter).

Keywords


fatty food fatty acids, liver, inflammation

Full Text:

PDF

References


Levitsky A. P. Idealnaya formula zhyrovogo pitaniya [The ideal formula of fatty food].Оdеssа, KP OGT, 2002: 64.

Kwaњniewski J. ywienie optymalne. 1999: 175.

De Pablo M. A., Alvarez de C. G. Modulatery effects of dietary lipids on immune system functions. Immunol. and Cell Biol. 2000; 78(1): 31-39. 4. Thors V. S., Pуrisdуrtit A., Erlendsdуttir H. [et al.]. The effect of dietary fish oil on survival after infection with Klebsiella pneumoniae or Streptococcus pneumoniae. Scand. J. Infec. Diseases. 2004; 36(2): 102-105.

Friedman M. I., Koch J. E., Graczyk-Milbrandt G. [et al.].Highfat diet prevents eating response and attennates liver ATP decline in rats given 2,5a-nhydro-D-mannitol. Amer. J. Physiol. 2002; 282(3), p. 2: R710-R714.

Zaldivar F., McMurray R. G., Nemet D. [et al.]. Body fat and circulating leukocytes in children. Int. J. Obesity. 2006; 30(6): 906- 911. 7. Ivashkin V. T., Maevskaya M. V. Lipotoxicity and metabolic disorders in obesity. Ross. zhurn. gastroenterol., gepatol., kopoproktol. 2010; 20(1): 13. 8. Borikov O. Yu., Gorbenko N. I. Вплив кверцетину на розвиток віщерального ожиріння та інсулінорезистентності у самців щурів за умов високожирової дієти. Fiziol. zhurn. 2010; 56(2): 128.

Bдckhed F., Ding H., Wang T. [et al.]. The gut microbiota as an environmental factor that regulates fat storage. Proc. Nat. Acad. Sci. USA. 2004; 101(44): 15718-15723.

Turnbangh P. J., Ley R. E., Mahowald M. A. [et al.]. An obesityassociated gut microbiome with increased capacity for energy harvest. Nature. 2006; 444(21/28): 1027-1031.

Bдckhed F., Manchester J. K., Semenkovich C. F. [et al.]. Mechanismus underlying the resistance to dietinduced obesity in germfree mice. Proc. Nat. Acad. Sci. USA. 2007; 104(3): 979-984.

Titov V. N. Высокое содержание пальмитиновой жирной кислоты в пище – основная причина повышения уровня холестерина липопротеинов низкой плотности и атероматоза интим артерий. Klin. labor. diagnostika. 2013; 2: 3-10. 13. Ambrosova T. N. Терапевтическая коррекция атерогенной дислипидемии при метаболическом синдроме. Mezhdun. med. zhurn. 2013; 3: 50-55.

Kravchun P., Shushlepin O., Mazhar S. S. Метаболический синдром: этиология, патогенез, клинические проявления. Liky Ukrainy. 2005; 2: 52-55.

Bondarenko V. M., Maleev V. V., Likhoded V. G. Intestinal microflora, obesity and type 2 diabetes. JMEI. 2014; 3: 42-49.

Ambroskina V. V., Kryachok T. A., Larionov O. P. [et al.] The presence and the characteristics of interrelation of disorders in metabolism of blood lipids and system inflammation. Fiziol. zhurnal. 2008; 54 (3): 36-46.

Velichko V. I., Tkachuk V. V., Levitsky A. P. Development of dysbiosis in tissues of rats fed with a high fat food. J. Health sciences. 2014; 4(12): 84-82.

Levitsky A. P. Olivka: unikalnoye podsolnechnoye maslo, analog olivkovogo [Olivka: the unique sunflower oil, the analogue to olive oil]. Odessa, KP OGT, 2013: 28.

Browning J. D., Horton J. D. Molecular mediators of hepatic steatoses and liver injury. J. Clin. Invest. 2004; 114(1): 147-152.

Levitsky A. P., Demyanenko S. A., Tsiselskiy Yu. V. Antimikrobnaya funktsiya pecheni [The antimicrobic function of liver].Оdеssа, KP OGT, 2011:141.

Levitsky A. P., Denga O. V., Makarenko O. A. [et al.]. Biokhimicheskie markery vospaleniya tkaney rotovoy polosti: metodicheskie rekomendatsii [Biochemical markers of inflammation of oral cavity tissue: method guidelines]. Оdеssа, KP OGT, 2010:16.

Stalnaya I. D., Garishvili T. G. Metod opredeleniya malonovogo dialdegida s pomoshchyu tiobarbiturovoy kisloty [The method of revelation of malonic dialdehyde with thiobarbituric acid]. Moskva, Meditsina, 1977: 66-68.

Levitsky A. P., Stefanov A. V. Metody opredeleniya aktivnosti elastazy i eye ingibitorov: metodicheskie rekomendatsii [The methods of the determination of the activity of elastase and its inhibitors: method guidelines]. Kiev, GFK, 2002: 15.

Girin S. V. The modification of the method of the determination of catalase activity in biological substrates. Laboratornaya diagnostika. 1999; 4: 45-46.

Levitsky A. P. Lizotsym vmesto antibiotikov [Lysozyme instead of antibiotics]. Оdеssа, KP OGT, 2005: 74.

Goryachkovskiy A. M. Klinicheskaya biokhimiya v laboratornoy diagnostike [The clinical biochemistry in laboratorial diagnostics] [3rd ed.]. Odessa, Ekologiya, 2005: 616.




DOI: http://dx.doi.org/10.5281/zenodo.1461947

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 © The Author (s) 2017

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

Journal Language(s): English 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2  www.ukw.edu.pl Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy http://ojs.ukw.edu.pl/index.php/johs  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase Index.com.

Website: www.infobaseindex.com

Universal Impact Factor 1.78 for year 2012. (http://www.uifactor.org/AppliedJournals.aspx)

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)