Expression of the initiator caspase-8 in the epithelium of physiological ectopy in young women, infected with Human papillomaviruses

A. O. Patskov


More and more often clinical cases of severe cervical lesions and cervical cancer are observed against the background of physiological ectopy at a young age. In most of them, Human papillomaviruses (HPVs) are detected at the examination, with severe intraepithelial lesions and initial forms of cancer identifying highly oncogenic strains of HPVs. The purpose of the study was to study the expression of the initiator caspase-8 in the epithelium of physiological ectopy in young women infected with HPVs. Material and methods. A randomized prospective cohort study was conducted on 180 patients aged 18-25 years, of which 150 were with physiological cervical ectopy and 30 without pathological changes in the cervix. The survey included extended colposcopy, HPV-screening, Pap-test, the quantitative determination of the level of human caspase-8 in lysates of cervical epithelial cells. Results. In patients with physiological ectopy of the cervix and the detection of high-oncogenic HPVs there was a low expression of caspase-8 (0.18 ± 0.01 ng/ml), which was less in comparison with the same in patients with physiological ectopy of the cervix and with low-oncogenic HPVs (1.72 ± 0.02 ng / ml) in 9.55 times, in women with physiological ectopy of the cervix and without infection of the HPVs (1.76±0.03 ng/ml) – in 9.78 times, in control group without physiological ectopy of cervical epithelium and without HPVs (1,78±0,04 ng/ml) – in 9,89 times. Conclusions. High-oncogenic HPV is actively involved in blocking apoptosis and reducing the activity of the enzyme initiating caspase-8, which leads to the disregulation of the cellular differentiation cycle, exfoliation, and disturbance of physiological metaplasia in the transformation zone.


physiological cervical ectopy, Human papillomaviruses, extended colposcopy, HPV-screening, Pap-test, caspase-8, physiological metaplasia, apoptosis

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Bruni L, Diaz M, Castellsague X, Ferrer E, Bosch FX, de Sanjose S. Cervical human papillomavirus prevalence in 5 continents: meta-analysis of 1 million women with normal cytological findings. J Infect Dis.2010 Dec 15;202(12):1789–99.

Cervix of the uterus, vagina, vulva. Physiology, pathology, colposcopy, aesthetic correction. Ed. SI Rogovskaya, EB Lipova. M.: StatusPraesens, 2016. 830 р.

Chatterjee K1, Williamson AL, Hoffman M, Dandara C. CASP8 promoter polymorphism is associated with high-risk HPV types and abnormal cytology but not with cervical cancer. J Med Virol. 2011 Apr;83(4):630-6. doi: 10.1002/jmv.22009.

Garnett TO, Duerksen-Hughes PJ. Modulation of apoptosis by human papillomavirus (HPV) oncoproteins. Arch Virol.2006;151:2321–2335.

Gewies A. Introduction to apoptosis. ApoReview. 2003:1–26.

Howie HL, Katzenellenbogen RA, Galloway DA. Papillomavirus E6 proteins. Virology. 2009;384:324–334.

Jiang P, Yue Y. Human papillomavirus oncoproteins and apoptosis (Review). Exp Ther Med. 2014 Jan; 7(1): 3–7. doi: 10.3892/etm.2013.1374.

Kaspersen MD, Larsen PB, Ingerslev HJ, et al. Identification of multiple HPV types on spermatozoa from human sperm donors.PLoS One. 2011;6:e18095.

Kharaeva ZF, Sizhazheva OA, De Luca C, Korkina LG. Ligands of apoptosis in papillomavirus-associated diseases of the cervix. Fundamental research. 2013; 3-2: 396-399.

M Olsson1 and B Zhivotovsky1,*Caspases and cancer. Cell Death Differ. 2011 Sep; 18(9): 1441–1449. Published online 2011 Apr 1. doi: 10.1038/cdd.2011.30.

Nichols AC, Palma DA, Chow W, et al. High frequency of activating PIK3CA mutations in human papillomavirus-positive orpharyngeal cancer. JAMA Otolaryngol Head Neck Surg.2013;139:617–622.

Prilepskaya VN, Rogovskaya SI, Kondrikov NI, Sukhikh G.T. Papillomavirus infection: diagnosis, treatment and prevention. М .: MEDpress-inform. 2007. 29 р.

Prilepskaya VN, Rudakova EB, Kononov AB. Ectopia and erosion of the cervix. М .: MEDpress-inform, 2002. 176 p.

Rogovskaya SI. Apoptosis in the pathology of the cervix, associated with the human papillomavirus. Access mode: ginec/01oct/ginec12/ginec.htm.

Severino A, Abbruzzese C, Manente L, et al. Human papillomavirus-16 E7 interacts with Siva-1 and modulates apoptosis in HaCaT human immortalized keratinocytes. J Cell Physiol.2007;212:118–125.

Yuan CH, Filippova M, Duerksen-Hughes P. Modulation of apoptotic pathways by human papillomaviruses (HPV): mechanisms and implications for therapy. Viruses. 2012;4:3831–3850.



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