Expression of the initiator caspase-8 in the epithelium of physiological ectopy in young women, infected with Human papillomaviruses

A. O. Patskov

Abstract


More and more often clinical cases of severe cervical lesions and cervical cancer are observed against the background of physiological ectopy at a young age. In most of them, Human papillomaviruses (HPVs) are detected at the examination, with severe intraepithelial lesions and initial forms of cancer identifying highly oncogenic strains of HPVs. The purpose of the study was to study the expression of the initiator caspase-8 in the epithelium of physiological ectopy in young women infected with HPVs. Material and methods. A randomized prospective cohort study was conducted on 180 patients aged 18-25 years, of which 150 were with physiological cervical ectopy and 30 without pathological changes in the cervix. The survey included extended colposcopy, HPV-screening, Pap-test, the quantitative determination of the level of human caspase-8 in lysates of cervical epithelial cells. Results. In patients with physiological ectopy of the cervix and the detection of high-oncogenic HPVs there was a low expression of caspase-8 (0.18 ± 0.01 ng/ml), which was less in comparison with the same in patients with physiological ectopy of the cervix and with low-oncogenic HPVs (1.72 ± 0.02 ng / ml) in 9.55 times, in women with physiological ectopy of the cervix and without infection of the HPVs (1.76±0.03 ng/ml) – in 9.78 times, in control group without physiological ectopy of cervical epithelium and without HPVs (1,78±0,04 ng/ml) – in 9,89 times. Conclusions. High-oncogenic HPV is actively involved in blocking apoptosis and reducing the activity of the enzyme initiating caspase-8, which leads to the disregulation of the cellular differentiation cycle, exfoliation, and disturbance of physiological metaplasia in the transformation zone.

Keywords


physiological cervical ectopy, Human papillomaviruses, extended colposcopy, HPV-screening, Pap-test, caspase-8, physiological metaplasia, apoptosis

Full Text:

PDF

References


Bruni L, Diaz M, Castellsague X, Ferrer E, Bosch FX, de Sanjose S. Cervical human papillomavirus prevalence in 5 continents: meta-analysis of 1 million women with normal cytological findings. J Infect Dis.2010 Dec 15;202(12):1789–99.

Cervix of the uterus, vagina, vulva. Physiology, pathology, colposcopy, aesthetic correction. Ed. SI Rogovskaya, EB Lipova. M.: StatusPraesens, 2016. 830 р.

Chatterjee K1, Williamson AL, Hoffman M, Dandara C. CASP8 promoter polymorphism is associated with high-risk HPV types and abnormal cytology but not with cervical cancer. J Med Virol. 2011 Apr;83(4):630-6. doi: 10.1002/jmv.22009.

Garnett TO, Duerksen-Hughes PJ. Modulation of apoptosis by human papillomavirus (HPV) oncoproteins. Arch Virol.2006;151:2321–2335.

Gewies A. Introduction to apoptosis. ApoReview. 2003:1–26.

Howie HL, Katzenellenbogen RA, Galloway DA. Papillomavirus E6 proteins. Virology. 2009;384:324–334.

Jiang P, Yue Y. Human papillomavirus oncoproteins and apoptosis (Review). Exp Ther Med. 2014 Jan; 7(1): 3–7. doi: 10.3892/etm.2013.1374.

Kaspersen MD, Larsen PB, Ingerslev HJ, et al. Identification of multiple HPV types on spermatozoa from human sperm donors.PLoS One. 2011;6:e18095.

Kharaeva ZF, Sizhazheva OA, De Luca C, Korkina LG. Ligands of apoptosis in papillomavirus-associated diseases of the cervix. Fundamental research. 2013; 3-2: 396-399.

M Olsson1 and B Zhivotovsky1,*Caspases and cancer. Cell Death Differ. 2011 Sep; 18(9): 1441–1449. Published online 2011 Apr 1. doi: 10.1038/cdd.2011.30.

Nichols AC, Palma DA, Chow W, et al. High frequency of activating PIK3CA mutations in human papillomavirus-positive orpharyngeal cancer. JAMA Otolaryngol Head Neck Surg.2013;139:617–622.

Prilepskaya VN, Rogovskaya SI, Kondrikov NI, Sukhikh G.T. Papillomavirus infection: diagnosis, treatment and prevention. М .: MEDpress-inform. 2007. 29 р.

Prilepskaya VN, Rudakova EB, Kononov AB. Ectopia and erosion of the cervix. М .: MEDpress-inform, 2002. 176 p.

Rogovskaya SI. Apoptosis in the pathology of the cervix, associated with the human papillomavirus. Access mode: http://www.nedug.ru/lib/lit/ ginec/01oct/ginec12/ginec.htm.

Severino A, Abbruzzese C, Manente L, et al. Human papillomavirus-16 E7 interacts with Siva-1 and modulates apoptosis in HaCaT human immortalized keratinocytes. J Cell Physiol.2007;212:118–125.

Yuan CH, Filippova M, Duerksen-Hughes P. Modulation of apoptotic pathways by human papillomaviruses (HPV): mechanisms and implications for therapy. Viruses. 2012;4:3831–3850.




DOI: http://dx.doi.org/10.5281/zenodo.1334468

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 © The Author (s) 2018

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

Journal Language(s): English 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2  www.ukw.edu.pl Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy http://ojs.ukw.edu.pl/index.php/johs  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase Index.com.

Website: www.infobaseindex.com

Universal Impact Factor 1.78 for year 2012. (http://www.uifactor.org/AppliedJournals.aspx)

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)