Experimental type 2 diabetes mellitus and acetaminophen toxic lesions: glutathione system indices changes

Olga Furka, Iryna Ivanusa, Mariya Mykhalkiv, Ivan Klishch

Abstract


Background. The goal of the research was to study the effect of acetaminophen on major glutathione part of antioxidant system indices in liver homogenate of rats with type 2 diabetes mellitus in time dynamics. Materials and methods. We conducted two series of experiments. In the first series toxic lesion was caused by a single intragastric administration of acetaminophen suspension in 2 % starch solution to animals in a dose of 1250 mg/kg (1/2 LD50). In the second series  the suspension of acetaminophen in 2 % starch solution in a dose of 55 mg/kg was given, which corresponds to the highest therapeutic dose during 7 days. Non-genetic form of experimental type 2 diabetes mellitus was modeled by Islam S., Choi H. method (2007). Activity of glutathione peroxidase (GPx) and glutathione reductase (GR), and contents of reduced glutathione (GSH) were determined in liver homogenate. Results. The obtained results have shown that GR and GPx activity actively decreased after acetaminophen administration in higher therapeutic doses to rats with type 2 DM. However, the changes were less pronounced than in rats with type 2 DM and acute acetaminophen toxic lesions. Conclusion. Results of the research have shown that acetaminophen administration to rats with type 2 DM causes a significant violation of compensatory mechanisms, especially of the enzyme and nonenzyme parts of antioxidant system.


Keywords


acetaminophen, reduced glutathione, glutathione peroxidase, glutathione reductase, diabetes mellitus

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References


Hlushko L. V. Vykorystannya paratsetamolu v kompleksnomu likuvanni khronichnoho pankreatytu z metoyu korektsiyi porushenʹ produktsiyi interleykinu-6 / L. V. Hlushko, I. T. Havrysh // Vrachebnoe delo.– 2005. – № 4. – S. 63–65.

Honsʹkyy YA.I., Mykhalkiv M.M. Vplyv histydynu na aktyvnistʹ deyakykh antyoksydantnykh fermentiv krovi tvaryn riznoho viku z khimichnym urazhennyam pechinky // Naukovyy visnyk CHNU. Seriya: Biolohiya. – 2001. – Vyp. 126. – S. 53-58.

Honsʹkyy YA.I., Mykhalkiv M.M. Vplyv histydynu ta yoho metalokompleksiv na stan hlutationovoyi systemy tvaryn riznoho viku z khimichnym urazhennyam pechinky // Naukovi zapysky Ter. derzh. ped. un-tu. Seriya: Biolohiya. - 2000. - № 4(11). - S. 63-67.

Endokrynolohiya. / Za red. prof. P.M. Bodnara.- K.: Zdorov'ya, 2002.-S. 207-302.

Ivanusa I. B. Vplyv atsetaminofenu na aktyvnistʹ protsesiv lipoperoksydatsiyi i stan antyoksydantnoyi systemy statevozrilykh shchuriv-samok na tli tryvaloho zastosuvannya prohestyniv ta estroheniv / I. B. Ivanusa, I. M. Klishch, I. Z. Kernychna, N. A. Vasylyshyn // Medychna khimiya. — Ternopilʹ, 2011. — № 2. — S. 56–62

Ivanusa I. B. Vplyv tiotryazolinu na deyaki pokaznyky antyoksydantnoyi systemy, vilʹnoradykalʹnoho okysnennya ta endohennoyi intoksykatsiyi u shchuriv z toksychnym urazhennyam atsetaminofenom na tli tryvaloho vvedennya estroheniv i prohestyniv / I. B. Ivanusa // Medychna khimiya. — Ternopilʹ, 2011. — № 3. — S. 75–80

Kozhemʺyakin YU.M. Naukovo-praktychni rekomendatsiyi z utrymannya laboratornykh tvaryn ta roboty z nymy / YU.M. Kozhemʺyakin, O.S. Khromov, M.A. Filonenko – K.: Avitsena, 2002. – 156 c.

Kruhlykova H.O. Hlutationperoksydazna ta hlutationreduktazna aktyvnistʹ pechinky shchuriv pislya vvedennya selenitu natriyu / H.O. Kruhlykova, TS.M. Shtutman // Ukr. biokhim. zhurn. – 1976. – T. 48, № 2. – S. 227–233.

Lapach S. N. Statystycheskye metody v medyko- byolohycheskykh yssledovanyyakh s yspolʹzovanyem Excel / S. N. Lapach, A. V. Chubenko, P. N. Babych. – K. : Moryon, 2000. – 320 s.

Ellman G. L.Tissue sulfhydryl groups // Arch. Bioch.Biophys. – 1959. - № 82. – Р. 70 – 77.

Acetaminophen Toxicity in an Urban County Hospital / F. V. Schiodt, F. A. Rochling, D. L. Casey, W. M. Lee / New England Journal of Medicine. – 1997. – Vol. 337, № 16. – Р. 1112–1117.

Bond G. R. Рopulation-based Incidence and Outcome of Acetaminophen Poisoning by Type of Ingestion / G. R. Bond, L. K. Hite // Academic Emergency Medicine. – 1999. – Vol. 6, № 11. – Р. 1115–1120.

Kobayashi H. Individual diversities in interferon gamma production by hyman peripheral blood mononuclear cells stimulated with periodontopathic bacteria / H. Kobayashi, T. Nagasawa, M. Aramaki // J. Periodontol. Res. – 2000. – Vol. 35. – N 6. – P. 319-328.




DOI: http://dx.doi.org/10.5281/zenodo.1231937

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