Biochemical and histochemical chenges in the activity of succinate dehydrogenase and ATP-ase in mitochondria of kidneys rats by acute intoxication

M. V. Dikal, Ye. O. Ferenchuk, T. M. Bilous, M. I. Grytsiuk, T. G. Kopchuk


Оbject of the study was to evaluate activities of succinate dehydrogenase and ATP-ase in mitochondria of kidney under conditions of toxic influence of 2,4-dinitrophenol. The experiment was conducted on 60 male albino rats. Toxic damage was caused by 0.1% solution of 2,4-dinitrophenol intraperitoneally in the dose of 3 mg / kg. The histochemical, biochemical and statistical methods of research were used. Mitochondria isolation was performed using the method of differential centrifugation. Determination of mitochondrial succinate dehydrogenase activity consists in the restoration of potassium ferricyanide (K3[Fe (CN)6]), whose solution has a yellow color, to the colorless ferricyanide of potassium (K4[F (CN)6]) succinate by the action of succinate dehydrogenase. The activity of ATP-ase was determined by the accumulation of inorganic phosphate.

The level of activity of succinate dehidrogenase was 39% lower than that of the control values. Whereas the level of activity of ATP-ase decreased by 48% in accordance with the control. Similar histochemical changes of the enzyme of the II complex of the mitochondrial respiratory chain were revealed.

The findings revealed that 2,4-dinitrophenol produced significant biochemical and histochemical changes in the kidney of the rats. This led to a decrease enzyme activities and as a result to a discrepancy between the energy needs of the cell and energy production in the system of mitochondrial oxidative phosphorylation.


mitochondria, kidney, 2,4-dinitrophenol, succinate dehydrogenase, ATP-ase activity

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Benard G, Bellance N, James D, Parrone P, Fernandez H, Letellier T & Rossignol R. Mitochondrial bioenergetics and structural network organization. J Cell Sci. 2007; 120: 838-48.

Elshennawy ATM, Sayed SR, et al. Histopathological and Histochemical Assessment of the Protective Effects of Zinc on Ethanol-Induced Acute Hepatotoxicity in Adult Albino Rats. J.Cytol. Histol. 2015; 6: 321.

Faccenda D. Molecular Regulation of the Mitochondrial F1F0-ATPsynthase: Physiological and Pathological Significance of the Inhibitory Factor 1 (IF1). Int. J. Cell Biol. 2012; 40 (12): 2698–701.

Gabibov MM. Influence of hyperbaric oxygenation on the activity of proton ATPase mitochondria of various tissues of rats. Ukr, biochem. journ. 1986; 58 (5): 68-71.

Gutman M, Silman N. The steady state activity of succinate dehydrogenase in the presence of opposing effectors.II. Reductive activation of succinate dehydrogenase in presence of oxaloacetate. Mol Cell Biochem 1975;7(3):177–185.

Hagerhall C. Succinate: quinone oxidoreductases. Variations on a conserved theme. Biochim Biophys Acta 1997;1320(2):107–141.

Hajjawi O.S.Succinate Dehydrogenase: Assembly, Regulation and Role in Human Disease. Eu. J. Sc. Res; 51 (1): 133 – 42.

King A, Selak MA, et al. Succinate dehydrogenase and fumarate hydratase: linking mitochondrial dysfunction and cancer. Oncogene 2006;25(34):4675–4682.

Mahran AA, Husam Eldien HO, Abd El-Mawla AMA, Attia AM. Protective effect of zinc (Zn) on the histology and histochemistry of liver and kidney of albino rat treated with cadmium. J Cytol Histol. 2011; (2):120 – 3.

Rogovy Yu.E., Belyavsky V.V., Dikal M.V. Modified proteins in the kidneys and the liver with intoxication with 2,4-dinitrophenol and the effects of melatonin in the experiment. Clin.Anat. and surgery. 2011; 10 (3): 18-21.

Sun F, Huo X, et al. Crystal structure of mitocho ndrial respiratory membrane protein complex II. Cell 2005;121(7):1043–1057.

Weinbach TC.A procedure for isolating stadle mitochondria from rat liver and kidney. Analyt.Biochem.1961; 2: 335-343.

Yankovskaya V, Horsefield R, et al. Architecture of succinate dehydrogenase and reactive oxygen species generation. Science 2003; 29 9(5607):700–704.



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