New diagnostic markers for the degree of liver fibrosis in children with chronic viral hepatitis

I. I. Nezgoda, L. V. Moroz, S. Singh, O. O. Singh


The article presents results of the study which includes 41 children with chronic viral hepatitis B (CHB) and acute lymphoblastic leukemia (ALL), aged 3 to 17 years and all were at dispensary observation in the Vinnytsia Regional Infectious Disease Hospital, Ukraine from January 2016 to February 2017. The aim of this study was to determine the plasma osteopontin level (OPN) in children, depending on sex, viral load, and degree of fibrosis. It was found that the level of this glycoprotein in children with chronic hepatitis B with ALL was significantly higher (275.66 ± 22.32 ng / ml) than in control group children (92.65 ± 7.31 ng / ml) (p <0, 05). The highest rates of OPN were found in younger children, with an increase in age, it decreases. In 68.24% of patients with CHB with ALL occurs with significant fibrotic changes in the liver: F1 fibrosis was found in 34.15% of patients, F2 in 31.7% of patients, F3 in 2.44% of patients. With the increase in the degree of fibrosis in the liver, the level of OPN increases. Its minimum value was found in children without fibrosis F0 (169.61 ± 21.19 ng / ml), with fibrosis F1 - this figure is (214.57 ± 14.49 ng / ml), with F2 - its level rises (432.39 ± 30.59 ng / ml), the maximum value of OPN was diagnosed in patients with fibrosis F3 (472.52 ng / ml). Direct correlation (r = 0.577, p <0.001) between the degree of fibrosis and the level of OPN was detected. Thus, osteopontin can be used as a new biomarker for the liver fibrosis.


chronic hepatitis B, children, lymphoblastic leukemia, fibrosis, osteopontin

Full Text:



Abeer M.N., Hala I.M. Hussien, Heba F.P., Naglaa A.M. (2011) Clinical Utility of Plasma Osteopontin in HCV-Related Chronic Liver Disease Patients // Medical journal of Cairo University, Vol. 79, No. 1, June 233-236.

Berezenko V.S. (2007). Kliniko-patohenetychni osoblyvosti fibrohenezu pechinky pry khronichnykh hepatytakh u ditey ta shlyakhy yoho medykamentoznoyi korektsiyi [Clinical and pathogenetic features of liver fibrogenesis in chronic hepatitis in children and ways of its medical correction]: Avtoref. dys. d-ra med. nauk. – 37p.

Berezenko V. S., Tsarʹova O. V. (2016). Kharakterystyka fibrozu pechinky u ditey z khronichnym virusnym hepatytom s za okremymy pryamymy ta nepryamymy markeramy [Characteristics of liver fibrosis in children with chronic viral hepatitis with separate direct and indirect markers] // Visnyk morfolohiyi, T. 22, № 1, p. 113-117.

Hind I. Fallatah (2014) Noninvasive Biomarkers of liver fibrosis: an overview. // Advances in Hepatology. – Vol. 2014. – 15p.

Huang W., Zhu G., Huang M., Lou G., Liu Y., Wang S. (2010) Plasma osteopontin concentration correlates with the severity of hepatic fibrosis and inflammation in HCV-infected subjects // Clin Chim Acta. – May 2; 411(9-10): 675-8.

Golubovska O. (2017) Local situation in Ukraine. // 3rd Central and Eastern European Meeting on Viral Hepatitis and Co-infection with HIV, 27-28 September 2017, Ljubljana, Slovenia / Reviews in Anviral therapy & Infectious Diseases 2017.

Kelleher T.B., Afdhal N. Noninvasive assessment of liver fibrosis (2005) // Clin. Liver Diseases. – Vol. 9, № 4. – p. 667-683.

Kramarev S.A., Doroshenko V.A., Voronov A.A., Yevtushenko V.V., Kilimnyuk G.I.,Shayda E.V., Agafonkina N.M. (2016). Virusnyye gepatity V i S u detey s onkogematologicheskoy patologiyey. [Viral hepatitis B and C in children with oncohematological pathology] // Aktual'naya infektologiya, № 1(10), p. 53 – 58.

Kryuchko T.O., Nesin I.M. (2012) Suchasni aspekty diahnostyky ta terapiyi khronichnoho virusnoho hepatytu pry virusniy etiolohiyi u ditey (Modern aspects of diagnosis and therapy of chronic viral hepatitis in viral etiology in children) / Dytyachyy likar. - № 8. - p. 29-33.

Mack C.L., Gonzalez-Peralta R.P., Gupta N. et al. (2012). NASPGHAN practice guidelines: Diagnosis and management of hepatitis C infection in infants, children, and adolescents // J. Pediatr Gastroenterol. Nutr., № 54 (6). – 838-55.

Pavlov C.S., Ivashkin V.T. (2007). Kak otsenit' i snizit' risk razvitiya fibroza, tsirroza i gepatotsellyulyarnoy kartsinomy u patsiyentov s khronicheskoy infektsiyey s gepatitom B i C (How to assess and reduce the risk of fibrosis, cirrhosis and hepatocellular carcinoma in patients with chronic infection with viruses hepatitis B and C) // ROS. zhurnal. gastroenterol., gepatol. i koloproktal'. - T. 17, № 5. - s. 16-24.

Pavlov, C.S. Fibroz pecheni s khronicheskim virusnym gepatitom V i S: avtoreferat. dis. Doktor med. Nauk (Fibrosis of the liver with chronic viral hepatitis B and C: author's abstract. dis. Dr. med. Sciences) / C.S. Pavlov. M., 2009. - 49s.

Pirogova I.Y. (2012) Diagnostika fibroza pecheni u patsiyentov s khronicheskim gepatitom V i S i yego klinicheskoye znacheniye: Avtoreferat dis. Doktor med. Nauk (Diagnosis of liver fibrosis in patients with chronic hepatitis B and C and its clinical significance : Author's abstract dis. Dr. med. Sciences) / I.Y. Pirogova, 2012 - 29 s.

Q. Ashton Acton (2013) Liver Fibrosis: New Insights for the Healthcare Professional: 2013 Edition. // Scholarly Editions. – 95p.

Samy A.H., Saleh S.A., Abdella H.M. (2015) Plasma osteopontin as a biomarker test in predicting liver fibrosis in Genotype 4 Chronic Hepatitis C infected patients // International Journal of Microbiology and Immunology Research Vol. 3(3), pp. 036-044.

Sase S.P., Ganu J.V., Nagane N. (2012) Osteopontin: a novel protein molecule // Indian Medical Gazette. – Feb. 2012. – p. 62-66.

Shadrin O.G., Magomedov O.O., Chernega N.F., Basaraba N.M., Dyukareva S.V., Kovalchuk A.A. (2014). Deyaki markery reheneratsiyi pechinky v ditey rannʹoho viku z urodzhenym i khronichnym hepatytom. [Some markers of regeneration of the liver in the early morning of the ugly creature and chronic hepatitis] // Zdorovʹe rebenka. - № 5, p. 7-12.

Shang S., Plymoth FA., Shaokui Ge, Feng Z., Rosen H.R., Sangrajrang S., Hainaut P., Marrero J.A., Baretta L. (2012) Identification of Osteopontin as a novel marker for early hepatocellular carcinoma // Hepatology. – Vol. 55, No. 2. – p. 483-490.

Trépo C., Chan H.L., Lok A. (2014) Hepatitis B virus infection // Lancet, 384 (9959), p. 2053-2063.

Weber G.F., Lett G.S., Haubein N.C. (2011) Categorial meta-analysis of osteopontin as a clinincal cancer marker // Oncology Reports 25: p. 433-441.

WHO Global hepatitis report, 2017.

Yazici O., Sendur M.A.N., Aksoy S. (2014). Hepatitis C virus reactivation in cancer patients in the era of targeted therapies // World J. Gastroenterol. № 20 (22). – p. 6716-24.

Ziol M., Handra-Luca A., Kettaneh A. et al. (2005) Non-invasive assessment of liver fibrosis by stiffness measurement: a prospective multicentre study in patients with chronic hepatitis С // Hepatology. – Vol. 41, № 1. – p. 48-54.



  • There are currently no refbacks.

Copyright (c) 2017 © The Author (s) 2017

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:

Journal Language(s): English 

PBN Poland



Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2 Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase


Universal Impact Factor 1.78 for year 2012. (

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski