The number and ratios of the metabolites of vitamin C - ascorbic, dehydroascorbic and diketogulonic acids were studied under the action of closed space hypoxia, acute blood loss and during sleep – the conditions associated with various oxygen saturation of the organism. It was found that in case of closed space hypoxia, the level of ascorbic and diketogulonic acid decreased with a simultaneous increase in the content of dehydroascorbic acid in the heart and brain. Acute blood loss resulted in decrease in the level of all metabolites of ascorbic acid. During sleep, the level of ascorbic acid metabolites increased. The ratio of vitamin-active metabolites to vitamin-inactive form of ascorbic acid in case of closed space hypoxia and acute blood loss decreased, and during sleep – it did not change significantly.

Figueroa-Méndez R, & Rivas-Arancibia S, Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain. Frontiers in Physiology, 2015;6:397.

Hacişevkđ A, An overview of ascorbic acid biochemistry. J. Fac. Pharm, 2009;38:3:233.

Guaiquil VH, Golde DW, Beckles DL, Mascareno EJ, Siddiqui MAQ, Vitamin c inhibits hypoxia-induced damage and apoptotic signaling pathways in cardiomyocytes and ischemic hearts. Free Radical Biol. Med., 2004;37:9:1419.

Patra RC, Swarup D, Dwivedi SK, Antioxidant effects of α tocopherol, ascorbic acid and l-methionine on lead induced oxidative stress to the liver, kidney and brain in rats. Toxicology, 2001;162:2:81.

De Tullio MC, Guether M, Balestrini R, Ascorbate oxidase is the potential conductor of a symphony of signaling pathways. Plant Signaling & Behavior, 2013;8:3:e23213.

Devi SA, Vani R, Subramanyam MVV, Reddy SS, Jeevaratnam K, Intermittent hypobaric hypoxia-induced oxidative stress in rat erythrocytes: protective effects of vitamin E, vitamin C, and carnitine. Cell Biochem. Funct., 2007:25:221.

Petrov SA, Budnyak AK, Сhernadchuk SS, Ozherelyeva KYu, Khmelnitska VV, Kravchuk IO, Sorokin AV, Regulation of lactate dehydrogenase activity by ascorbic acid in organs of rats with hypoxia of closed space. ScienceRise, 2015;7:1(12):7. (in Ukrainian)

Arad I, Sidi A, Shohami E, Effect of Acute Hypoxia on Ascorbate Content of Plasma, Cerebral Cortex, and Adrenal Gland. J. Neurochem., 1985;45:766.

Karkischenko NN, Rukovodstvo po laboratornym zhivotnym i al'ternativnym modeljam biomedicinskih tehnologij [Guide to laboratory animals and alternative models of biomedical technologies], Moscow; 2010;344. (in Russian)

Sokolovsky VV, Lebedeva LV, Lijelup TV, A separate method of determining of ascorbic, dehydroascorbic and diketogulonic acids in biological tissues. Laboratornoe delo, 1974;3:160. (in Russian)

Glants, S. Mediko-biologicheskaja statistika [Biomedical Statistics], Moscow: Practika; 1998; 459.

Directive 2010/63/EU of the European parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC.20.10.2010. European Union Off. J. Eur. Communities: Legis; 2010;53:33.

Сhernadchuk SS, A. O. G. Rustamova AOG, Budnyak AK, Petrov SA, State of free-radical peroxidation processes in rats with acute blood loss. ScienceRise, 2015;4:1(9):16. (in Russian).