Morpho-functional basis of endothelial dysfunction in diabetes mellitus

A. I. Gozhenko, H. S. Kuznetsova, K. S. Kuznetsova, O. M. Kuznetsova, T. M. Byts, W. Zukow


The diabetes mellitus (DM) inevitably progresses and leads to complications, among which the main place is occupied by micro- and macroangiopathies. The presence of endothelial damage in DM can be established even before macroscopically significant damage to the vessel. At the same time, there is no summary ED characteristic for diabetes. The aim of the study is to make a comprehensive evaluation of ED in DM -1 and DM-2 types.

Materials and methods of research. 60 persons, including 53 DM type 1 and type 2, with a severe course (state of decompensation) participated in the present study. We used the method of estimating ED by the number of circulating desquamation endothelial cells (CECs) at the stages of decomposition with simultaneous determination of NO2- and NO3- metabolites of nitric oxide.

Results and discussion. In patients with diabetes, the level of CECs increased in 3-5 times and ranged from 1800 to 11,200 cells / ml. The average amount of CECs in patients with diabetes was 3358.5 ± 366.3 cells / ml.

Conclusions: Endothelium is involved in the pathological process at DM. This is evidenced by a significant increase in CECs in the blood plasma. The use of this method allows to detect ED before clinically considerable vascular impairment and reflects the severity of the course and duration of DM.


diabetes mellitus, endothelial dysfunction, circulating endothelial cells, vascular endothelium, nitric oxide

Full Text:



Global report on diabetes. WHO, 2016.

Kuznetsova ES, Kuznetsova AS, Shukhtin VV, Gozhenko AI. Osobennosti osmoreguliruyushchey funktsii pochek u bol'nykh s sakharnym diabetom 2 tipa [Features of osmoregulatory renal function in patients with type 2 diabetes]. Ukrainian Journal of Nephrology and Dialysis, 2015;4(49):21-26. (in Russian)

Petrischeva NN. Disfunktsiya endoteliya. Prichiny, mekhanizmy, farmakologicheskaya korrektsiya [Endothelian Disfunction. Causes, mechanisms, pharmacological correction], SpB: St. Petersburg State Medical University; 2003:184. (in Russian)

Malaya LT, Korzh AN, Balkova LB. Endotelial'naya disfunktsiya pri patologii serdechno- sosudistoy sistemy [Endothelial dysfunction in the pathology of the cardiovascular system], Kharkov: Foliant; 2000;432. (in Russian)

Shishkin AN, Lyndina ML. Endotelial'naya disfunktsiya i arterial'naya gipertenziya [Endothelial dysfunction and hypertension]. Arterial'naya gipertenziya, 2008;14(4): 315-319. (in Russian)

Kotyuzhinskaya SG, Gozhenko AI. Kharakteristika lipidtransportnoy sistemy u bol'nykh pri gipogeparinemii [Characteristics of the lipid transport system in patients with hypogaparinemia]. Bukovins'kiy medichniy vísnik, 2014;18(2):57-59.

Gomazkov OA. Endoteliy — endokrinnoye derevo [Endothelium-endocrine tree]. Priroda, 2000;5:38-46. (in Russian)

Lusсher TF, Barton M. Biology of the endothelium. Clin. Cardiology, 1997;20:310.

Kirichuk VF, Rebrov AP, Rossoshanskaya SI. Funktsii endoteliya sosudistoy stenki [Endothelial functions of the vascular wall] Tromboz, gemostaz, reologiya, 2005; 2:23-29. (in Russian)

Zateyshchikova AA, Zateyshchikov DA. Endotelial'naya regulyatsiya sosudistogo tonusa: metody issledovaniya i klinicheskoye znacheniye [Endothelial regulation of vascular tone: research methods and clinical significance]. Kardiologiya, 1998;9:68-80. (in Russian)

Inagami T, Naruse M, Hoover R. Endothelium as an endocrine organ. Annu.Rev. Physiol, 1995; 57: 171-189.

Duvall WL. Endothelial dysfunction and antioxidants Mt. Sinai J.Med., 2005;72(2):71-80.

Tret'yakov SV, Shpagina LA. Strukturno–funktsional'noye sostoyaniye serdechno –sosudistoy sistemy u lits pozhilogo i starcheskogo vozrasta [Structurally functional state of the cardiovascular system in persons of elderly and senile age]. Klinicheskaya gerontologiya, 2006;10:23-28. (in Russian)

Greenwald SE. Aging of the conduit arteries. J. Pathol, 2007; 211(2): 157-172.

Jani B, Rajkumar C. Ageing and vascular ageing. Postgrad Med J, 2006;82(967):357-362.

Vishnevskaya VY. Endotelial'naya disfunktsiya i vozrast [Endothelial dysfunction and age]. Vrachebnaya praktika, 2003;4:5-10. (in Russian)

Gorshunova NK. Strukturnyye i funktsional'nyye osobennosti mikrotsirkulyatornogo zvena gemostaza pri starenii [Structural and functional features of the microcirculatory component of hemostasis in aging]. Mater. Vseross. konf. Aktual'nyye problemy professional'noy i ekologicheskoy patologii. Kursk, 1994;252-257. (in Russian)

Ostroumova OD, Dubinskaya RE. Stareniye i disfunktsiya endoteliya [Aging and dysfunction of the endothelium]. Kardiovaskulyarnaya terapiya i profilaktika, 2004;4:83-89. (in Russian)

Bulpitt CJ, Rajkumar C, Cameron JD. Vascular compliance as a measure of biological age. J. Am. Geriat. Soc, 1999;47:657-663.

Hinderliter AL, Caughey M. Assessing endothelial function as a risk factor for cardiovascular disease. Curr. Atheroscler. Rep., 2003;5(6):506-513.

Storey AM, Perry CJ, Petrie JR. Endothelial dysfunction in type 2 diabetes. The british j. of diabetes and vascular disease, 2001;1(l):22-27.

Tadtayeva NY, Basiyeva OO.Diagnosticheskoye znacheniye markerov povrezhdeniya endoteliya sosudov pri sakharnom diabete 2-go tipav sochetanii s arterial'noy gipertenziyey [Diagnostic value of markers of vascular endothelium damage in type 2 diabetes mellitus combined with arterial hypertension]. Kubanskiy nauchnyy meditsinskiy vestnik, 2014;3(145):109-113. (in Russian)

Petrishchev NN, Berkovich OA, Vlasov TD. Diagnosticheskaya tsennost' opredeleniya deskvamirovannykh endotelial'nykh kletok v krovi [Diagnostic value of the definition of desquamated endothelial cells in the blood]. Klinicheskaya laboratornaya diagnostika, 2001,1:50-52. (in Russian).

Susla OB, Misula IR. Pat. 57841 Ukraí̈na, MPK A61V 10/00 Sposib viznachennya vmistu tsirkulyuyuchikh yendotelial'nikh klitin u plazmi krovi [Method for determining the content of circulating endothelial cells in the blood plasma]; zayavnik i patentovlasnik Derzhavniy vishchiy navchal'niy zaklad Ternopil's'kiy derzhavniy medichniy universitet imeni I. YA. Gorbachevs'kogo, № 2010 11243 ; zayavl. 20.09.2010 ; opubl. 10.03.2011,5. (in Ukranian)

Kuznetsova AS, Kvasnevskaya NF, Kuznetsova ES, Byts TN, Gozhenko AI.. Morpho-functional state of endothelium in diabetes mellitus. Current issues of development of new drugs, abstracts XXIV International scientific conference of young scientists and students (20 April 2017). 2017;2:84.

Taddei S, Virdis A, Ghiadoni L. Aging and endothelial function in normotensive subjects and patients with essential hypertension. Circulation, 1995;91:1981-1987.

Kobylyanskiy AG, Kuznetsova TV, Soboleva GN. Opredeleniye oksida azota v syvorotke i plazme krovi cheloveka. Metod vysokoeffektivnoy zhidkostnoy khromatografii [Determination of nitric oxide in the serum and plasma of the human blood. Method of high-efficient liquid chromatography]. Biomeditsinskaya khimiya, 2003;49(6):597-603. (in Russian)

Mauyer SS. Involyutivnaya vazotonicheskaya endotelial'naya disfunktsiya, yeyo osobennosti [Involute vasotonic endothelial dysfunction, its features]. Mater. IV Mezhd.nauch. konferentsiya molodykh uchenykh medikov, Kursk,2010;11:272-274. (in Russian)



  • There are currently no refbacks.

Copyright (c) 2017 © The Author (s) 2017

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:

Journal Language(s): English 

PBN Poland



Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2 Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase


Universal Impact Factor 1.78 for year 2012. (

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski