Variety of immune responses to chronic stress in rats male

Іlona S Polovynko, Walery Zukow

Abstract


Background. Previously we have been carry out integrated quantitative estimation of neuroendocrine and immune responses to chronic restraint stress in male rats. Revealed that the value of canonical discriminant roots rats subjected to chronic stress different not only on the values of intact animals (by definition), but also among themselves. So we set a goal retrospectively divided stressed rats into three homogeneous groups. Material and methods. The experiment is at 50 white male rats. Of these 10 animals not subjected to any influences and 40 within 7 days subjected to moderate stress by daily 30-minute immobilization. The day after the completion of stressing in portion of the blood immunological parameters were determined by tests I and II levels of WHO. The spleen and thymus did smears for counting spleno- and thymocytograms. Results. The method of cluster analysis (k-means clustering) formed three groups-clusters. For further analysis selected 18 parameters that members of each cluster differing minimum and maximum are different from members of other clusters (η2=0,73÷0,15; F=49,0÷3,26; p=10-6÷0,05). We stated that in 16 rats from cluster III the deviation 16 parameters in either side of the average norm almost identical and are in an acceptable range of ±0,5σ. Thus, the immune status of 40% of the rats subjected to moderate chronic stress was resistant to its factors. For the immune status of the 15 (37,5%) rats cluster II typical moderate inhibition microphage, killer and T-cellular links in combination with a strong activation macrophage link. Poststressory changes in immunity in 9 rats (22,5%) from cluster I differ from those in cluster II both qualitatively and quantitatively. In particular, the rats in this cluster were found no deviations from the norm or reaction blast transformation T-cells nor NK-lymphocytes levels. However, other parameters of T-link and microhage link suppressed more and settings macrophage link appeared activated very significantly, and the area of activation, except thymus and blood, spread to the spleen. Conclusion. We assume that a variety of immune responses to chronic stress caused by a variety of reactions neuroendocrine factors.


Keywords


chronic stress, thymocytogram, splenocytogram, immunocytogram of blood, male rats.

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References


Aldenderfer MS, Blashfield RK. Cluster analysis (Second printing, 1985) [trans. from English in Russian]. In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika. 1989: 139-214.

Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva: Nauka. 1984. 221 p.

Babylyuk RV, Popovych IL. Influence of bioactive water Naftussya on resistance to hypoxic hypoxia and stressinduced changes to mucous membrane of stomach, ECG and leukocytogram at rats [in Ukrainian]. Medical Hydrology and Rehabilitation. 2011; 9(3): 45-59.

Barylyak LG, Babylyuk RV, Popovych IL, Korolyshyn TA, Nesterova LF. Influence of balneotherapy on spa Truskavets on resistance to hypoxia for children with dysfunction of neuroendocrine-immune complex [in Ukrainian]. Medical Hydrology and Rehabilitation. 2011; 9(4): 4-38.

Barylyak LG, Fil’ VM, Romans’kyi IYu, Tkachuk SP, Bilyns’ka GI. Resistance to hypoxia and neuroendocrine-immune complex and metabolism in children who arrive at Truskavets from areas contaminated with radionuclides [in Ukrainian]. Medical Hydrology and Rehabilitation. 2010; 8(4): 10-21.

Bazarnova MA. Cytology investigation punctates spleen. In: Guide to practical training in clinical laboratory diagnostics [in Russian]. Kyiv: Vyshcha shkola. 1988: 263-264.

Berezovskyi VYa (editor). Hypoxia and individual features of reactivity [in Russian]. Kyiv: Naukova dumka, 1978. 216 p.

Bianco C. Population of lymphocytes bearing a membrane receptor for antigen-antibody complex. J Exp Med. 1970; 134(4): 702-720.

Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.

Chrousos GP. The stress response and immune function: clinical implications. The 1999 Novera H Spector lecture. Neuroimmunomodulation. Perspectives at the new millenium. ANYAS. 2000; 917: 38-67.

Dhabhar FS. Enhancing versus Suppressive Effects of Stress on Immune Function: Implications for Immunoprotection and Immunopathology. Neuroimmunomodulation. 2009; 16(5): 300–317.

Douglas SD, Quie PG. Investigation of Phagocytes in Disease. Churchil. 1981. 110 p.

Garkavi LKh, Kvakina YeB, Kuz’menko TS. Antistress Reactions and Activation Therapy [in Russian]. Мoskva: Imedis, 1998. 654 p.

Gozhenko AI, Hrytsak YL, Barylyak LG, Kovbasnyuk MM, Tkachuk SP, Korolyshyn TA, Matiyishyn GY, Zukow W, Popovych IL. Features of immunity by various constellations of principal adaptation hormones and autonomous regulation in practically healthy people. Journal of Education, Health and Sport. 2016; 6(10). 215-235.

Hrytsak YaL, Barylyak LG, Zukow W, Popovych IL. Cluster analysis of hormonal constellation at women and men with harmonious and disharmonious general adaptation reactions. Journal of Education, Health and Sport. 2016; 6(4): 141-150.

Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2): 207-215.

Khaitov RM. Physiology of immune system [in Russian]. Moskva: VINITI RAN. 2005. 428 p.

Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika. 1989: 78-138.

Kolyada TI, Volyanskyi YL, Vasilyev NV, Maltsev VI. Adaptation Syndrome and Immunity [in Russian]. Kharkiv: Osnova. 1995. 168 p.

Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2015. 349 p.

Lapovets’ LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. Lviv. 2002. 173 p.

Limatibul S, Shore A, Dosch HM, Gelfand EW. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. Clin Exp Immunol. 1978; 33(3): 503-513.

Lukyanchenko OI. Multivariate effects on gastric mucosa acute water-immersion stress and their neuroendocrine, metabolic and immune support [in Ukrainian]. Medical Hydrology and Rehabilitation. 2008; 6(2): 55-71.

Lukyanchenko OI. Features poststressory changes of neuroendocrine, metabolic and immune parameters in rats with differently severe injuries gastric mucosa [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 119-125.

Lukyanchenko OI. Features exchange gastrin and gastric secretion in rats of different states of the stomach after ligation of the pylory [in Ukrainian]. Taurian biomedical messenger. 2011; 14(4,Pt1): 123-127.

Lukyanchenko OI, Budnyk OM, Popovych IL. Prediction of individual resistance of gastric mucosa and myocardium of rats to stress damage [in Ukrainian]. In: Mater. VI scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil, October 31-November 1, 2013). Achievements of Clinical and Experimental Medicine. 2013; 2(19): 260.

Markova OO, Popovych IL, Tserkovnyuk AV, Barylyak LG. Adrenaline myocardiodystrophy and reactivity of organism [in Ukrainian]. Kyiv: Computerpress, 1997. 126 p.

Polovynko IS, Popovych IL. Immunotropic traits in responses to chronic stress rats males [in Ukrainian]. In: VV Podvysotskiy XI reading. Bulletin Materials Science Conference (Odesa, 24-25 May, 2012). Odessa: Ukrainian Research Institute of Transport Medicine, 2012: 109-110.

Polovynko IS, Popovych IL. Variety immunotropic responses to chronic stress and neuroendocrine accompaniment of male rats [in Ukrainian]. In: Mater. V scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil, November 1-2, 2012). Achievements of Clinical and Experimental Medicine. 2012; 2(17): 200.

Polovynko IS, Zajats LM, Popovych IL. Neuroendocrine-immune relationships in males rats in conditions of chronic stress [in Ukrainian]. In: Mater. VI scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil, October 31-November 1, 2013). Achievements of Clinical and Experimental Medicine. 2013; 2(19): 274.

Polovynko IS, Zajats LM, Popovych AI, Popovych IL. Іntegral quantification of neuroendocrine and immune responses to chronic stress in male rats [in Ukrainian]. In: Pathophysiology and Pharmacy: ways of integration: Abstracts VII National Congress pathophysiologists Ukraine with international participation (5-7 October 2016). Kharkiv: NPhU, 2016: 182.

Polovynko IS, Zayats LM, Zukow W, Popovych IL. Neuro-endocrine-immune relationships by chronic stress at male rats. Journal of Health Sciences. 2013; 3(12): 365-374.

Polovynko IS, Zajats LM, Zukow W, Yanchij RI, Popovych IL. Quantitative evaluation of integrated neuro-endocrine and immune responses to chronic stress rats male. Journal of Education, Health and Sport. 2016; 6(8): 154-166.

Popovych IL. Stresslimiting Adaptogenic Mechanisms of Biological and Therapeutic Activity of Water Naftussya [in Ukrainian]. Kyiv: Computerpress. 2011. 300 p.

Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-87.

Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18.

Popovych IL, Ivassivka SV, Barylyak LG, Fil’ VM, Korolyshyn TA, Shologin AI, Dats’ko OR. Peculiarities of stressinduced changes of mucous stomach, neuro-endocrine-immune complex and metabolism in rats with various resistance to hypoxia [in Ukrainian]. Medical Hydrology and Rehabilitation. 2010; 8(2): 96-109.

Popovych IL, Ivassivka SV, Yassevych AP et al. The protective effect of organic matter in the water Naftusya on erosive and ulcerative lesions of the gastric mucosa at rats under immobilization-cold stress [in Russian]. Fiziol Zhurn. 1990; 36(4). 68-76.

Popovych IL, Vis’tak HI, Gumega MD, Ruzhylo SV. Vegetotropic Effects of Bioactive Water Naftussya and their Endocrine-Immune, Metabolic and Hemodynamic Accompaniments [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2014. 163 p.

Popovych IL, Zajats LM, Polovynko IS, Lukyanchenko ОІ. Traits in response to chronic stress some immune and psycho-physiological parameters children [in Ukrainian]. In: “Actual problems of biophysical medicine”. Materials VII International symposium (Kyiv, 14-17 May 201). Кyiv: OO Bohomolets’ Institute of Physiology, 2014: 104-105.

Schauenstein K, Felsner P, Rinner I, Liebmann PM, Stevenson JR, Westermann J, Haas HS, Cohen RL, Chambers DA. In vivo immunomodulation by peripheral adrenergic and cholinergic agonists/antagonists in rat and mouse models. Neuroimmunomodulation. Perspectives at the new millenium. ANYAS. 2000; 917: 618-627.

Sternberg EM. Neural regulation of innate immunity: a coordinated nonspecific host response to pathogens. Nat Rev Immunol. 2006; 6(4): 318-328.

Sydoruk NO, Zukow W, Yanchij RI. Integrated quantitative assessment of changes in neuro-endocrine-immune complex and metabolism in rats exposed to acute cold-immobilization stress. Journal of Education, Health and Sport. 2016; 6(9): 724-735.

Thayer JF, Sternberg EM. Neural aspects of immunomodulation: Focus on the vagus nerve. Brain Behav Immun. 2010; 24(8): 1223-1228.

Tracey KJ. Reflex control of immunity. Nat Rev Immunol. 2009; 9(6): 418-428.

Uchakin PN, Uchakina ON, Tobin BV, Ershov FI. Neuroendocrine immunomodulation [in Russian]. Vestnik Ross AMN. 2007; 9: 26-32.

Vis’tak HI, Popovych IL. Vegetotropic effects of bioactive water Naftussya and their endocrine and immune support in female rats [in Ukrainian]. Medical Hydrology and Rehabilitation. 2011; 9(2): 39-57.

Yushkovs’ka OG. Using information theory to study adaptive responses in the body athletes [in Ukrainian]. Medical Rehabilitation, Kurortology, Physiotherapy. 2001; 1(25): 40-43.




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