Modulating effects of bioactive water Naftussya from layers Truskavets’ and Pomyarky on neuro-endocrine-immune complex and metabolism at rats exposed to acute stress

Nadiya O Sydoruk, Anatoliy I Gozhenko, Walery Zukow


Background. Previously we have been carry out integrated quantitative estimation of neuroendocrine, immune and metabolic responses to acute stress at male and female rats. The aim of this study is to compare the modulating effects of Bioactive Water Naftussya (BAWN) from layers Truskavets’ and Pomyarky on neuro-endocrine-immune complex and metabolism at rats exposed to acute stress. Materials and methods. The experiment is at white rats Wistar line. Rats were divided into intact and 5 test groups treated for seven days with tap (control) water, distilled water as well as table water "Truskavets’ka" and BAWN from Truskavets’ and Pomyarky layers. A day after the end of course animals test groups subjected to water immersion restraint stress. A day after stress determined HRV, endocrine, immune and metabolic parameters as well as gastric mucosa injuries and comparing them with parameters of intact animals. Results. BAWN both Truskavets’ and Pomyarky layers minimizes or obviates abnormal 15 parameters caused by stress, but does not affect the poststressory deviation of 7 parameters. On the other hand, BAWN causes deviation of 15 other parameters which little or do not change under stress. Distilled water and water "Truskavets’ka" affect some parameters the opposite way, other less favorable, but some more favorably than BAWN. Conclusion. BAWN both Truskavets’ and Pomyarky layers limit pathogenic effects of stress, and initiate or enhance its compensatory and sanogenic effects.


acute stress, HRV, hormones, immunity, metabolism, rats, bioactive water Naftussya.

Full Text:



Andreyeva LI, Kozhemyakin LA, Kishkun AA. Modification of the method for determining the lipid peroxide in the test with thiobarbituric acid [in Russian]. Laboratornoye Delo. 1988; 11: 41-43.

Avtandilov GG. Medical Morphometry [in Russian]. Moskva: Meditsina. 1990. 384 p.

Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva: Nauka. 1984. 221 p.

Bazarnova MA. Cytology investigation punctates spleen. In: Guide to practical training in clinical laboratory diagnostics [in Russian]. Kyiv: Vyshcha shkola. 1988. 263-264.

Bianco C. Population of lymphocytes bearing a membrane receptor for antigen-antibody complex. J Exp Med. 1970; 134(4): 702-720.

Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.

Chrousos GP. The stress response and immune function: clinical implications. The 1999 Novera H Spector lecture. Neuroimmunomodulation. Perspectives at the new millenium. ANYAS. 2000; 917: 38-67.

Dhabhar FS. Enhancing versus Suppressive Effects of Stress on Immune Function: Implications for Immunoprotection and Immunopathology. Neuroimmunomodulation. 2009; 16(5): 300–317.

Douglas SD, Quie PG. Investigation of Phagocytes in Disease. Churchil. 1981. 110 p.

Dubinina YY, Yefimova LF, Sofronova LN, Geronimus AL. Comparative analysis of the activity of superoxide dismutase and catalase of erythrocytes and whole blood from newborn children with chronic hypoxia [in Russian]. Laboratornoye Delo. 1988; 8: 16-19.

Fajda OI, Drach OV, Barylyak LG, Zukow W. Relationships between Ca/K plasma ratio and parameters of Heart Rate Variability at patients with diathesis urica. Journal of Education, Health and Sport. 2016; 6(1): 295-301.

Garkavi LKh, Kvakina YeB, Kuz’menko TS. Antistress Reactions and Activation Therapy [in Russian]. Мoskva: Imedis, 1998. 654 p.

Gavrilov VB, Mishkorudnaya MI. Spectrophotometric determination of plasma levels of lipid hydroperoxides [in Russian]. Laboratornoye Delo. 1983; 3: 33-36.

Goryachkovskiy AM. Clinical biochemi [in Russian]. Odesa: Astroprint. 1998. 608 p.

Gozhenko AI, Hrytsak YL, Barylyak LG, Kovbasnyuk MM, Tkachuk SP, KorolyshynTA, Matiyishyn GY, Zukow W, Popovych IL. Features of immunity by various constellations of principal adaptation hormones and autonomous regulation in practically healthy people. Journal of Education, Health and Sport. 2016; 6(10). 215-235.

Hrytsak YaL, Barylyak LG, Zukow W, Popovych IL. Cluster analysis of hormonal constellation at women and men with harmonious and disharmonious general adaptation reactions. Journal of Education, Health and Sport. 2016; 6(4): 141-150.

Instructions for application for recruitment reagents for ELISA investigations hormones in the blood of humans [in Russian]. St. Petersburg: JSC "Alkor Bio". 2000.

Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2): 207-215.

Khaitov RM. Physiology of immune system [in Russian]. Moskva: VINITI RAN. 2005. 428 p.

Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika. 1989: 78-138.

Kolyada TI, Volyanskyi YL, Vasilyev NV, Maltsev VI. Adaptation Syndrome and Immunity [in Russian]. Kharkiv: Osnova. 1995. 168 p.

Korolyuk MA, Ivanova MI, Mayorova IG, Tokarev VYe. The method for determining the activity of catalase [in Russian]. Laboratornoye Delo. 1988; 1: 16-19.

Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2015. 349 p.

Lapovets’ LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. Lviv. 2002. 173 p.

Левкут ЛГ, Алєксєєв ОІ, Попович ІЛ. Стреслімітуюча дія деяких ксенобіотиків та адаптогенів. В кн.: Проблеми патології в експерименті та клініці: Наук. роботи Дрогобицького мед. ін-ту. Т. XV. Дрогобич, 1994. 23-25. Levkut LG, Alyeksyeyev OI, Popovych IL. Stresslimiting action of some xenobiotics and adaptogens [in Ukrainian]. In: Problems pathology in experiment and clinic. Scient. Publ. Drohobych Medical Inst. Vol. XV. Drohobych. 1994. 23-25.

Limatibul S, Shore A, Dosch HM, Gelfand EW. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. Clin Exp Immunol. 1978; 33(3): 503-513.

Makarenko YeV. A comprehensive definition of the activity of superoxide dismutase and glutathione reductase in red blood cells in patients with chronic liver disease [in Russian]. Laboratornoye Delo. 1988; 11: 48-50.

Nakamura J, Takada S, Ohtsuka N, Heya T et al. An assessment of gastric ulcers in vivo: enhancement of urinary recovery after oral administration of phenolsulfonphtalein in rats. J Parm Dyn. 1984; 7(7): 485-491.

Nance DM, Sanders VM. Autonomic innervation and regulation of the immune system. Brain Behav Immun. 2007; 21(6): 736-745.

Панасюк ЄМ, Левкут ЛГ, Попович ІЛ. та ін. Експериментальне дослідження адаптогенних властивостей бальзаму "Кримський". Фізіол. журн. 1994; 40(3-4): 25-30. Panasyuk YM, Levkut LG, Popovych IL et al. Experimental study adaptogenic properties balm "Kryms’kyi" [in Ukrainian]. Fiziol Zhurn. 1994; 40(3-4): 25-30.

Polovynko IS, Zayats LM, Zukow W, Popovych IL. Neuro-endocrine-immune relationships by chronic stress at male rats. Journal of Health Sciences. 2013; 3(12): 365-374.

Polovynko IS, Zajats LM, Zukow W, Yanchij RI, Popovych IL. Quantitative evaluation of integrated neuro-endocrine and immune responses to chronic stress rats male. Journal of Education, Health and Sport. 2016; 6(8): 154-166.

Popоvych IL. The factor and canonical analysis parameters of neuro-endocrine-immune complex, metabolism and erosive-ulcerose injuries of mucous stomach at rats in conditions of acute water іmmersing stress [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(2): 68-80.

Popоvych IL. Information effects of bioactive water Naftyssya in rats: modulation entropic, prevention desynchronizing and limitation of disharmonizing actions water immersion stress for information components of neuro-endocrine-immune system and metabolism, which correlates with gastroprotective effect [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(3): 50-70.

Popоvych IL. Bioactive water Naftyssya in general like ginseng limits, negates, reverses neuro-hormonal, metabolic and immune pathogens manifestations and strengthens sanogens manifestations acute stress in rats without impacting significantly on performance, not subordinates stress action [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(4): 7-29.

Popovych IL. Stresslimiting Adaptogenic Mechanisms of Biological and Therapeutic Activity of Water Naftussya [in Ukrainian]. Kyiv: Computerpress. 2011. 300 p.

Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-87.

Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18.

Попович ИЛ, Ивасивка СВ, Ясевич АП. и др. Защитное действие органических веществ воды нафтуся на эрозивно-язвенные повреждения слизистой оболочки желудка у крыс при иммобилизационно-холодовом стрессе. Физиол. журн. 1990; 36(4). 68-76. Popovych IL, Ivassivka SV, Yassevych AP et al. The protective effect of organic matter in the water Naftusya on erosive and ulcerative lesions of the gastric mucosa at rats under immobilization-cold stress [in Russian]. Fiziol Zhurn. 1990; 36(4). 68-76.

Popovych IL, Vis’tak HI, Gumega MD, Ruzhylo SV. Vegetotropic Effects of Bioactive Water Naftussya and their Endocrine-Immune, Metabolic and Hemodynamic Accompaniments [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2014. 163 p.

Schauenstein K, Felsner P, Rinner I, Liebmann PM, Stevenson JR, Westermann J, Haas HS, Cohen RL, Chambers DA. In vivo immunomodulation by peripheral adrenergic and cholinergic agonists/antagonists in rat and mouse models. Neuroimmunomodulation. Perspectives at the new millenium. ANYAS. 2000; 917: 618-627.

Shatalov VN, Polonskiy VM, Bulgakov SA, Vinogradov VA. The ligands of opiate receptors and experimental ulcer. In: Modern methods of diagnosis and treatment of internal diseases [in Russian]. Collection of Scientific Papers IV Main Administration under the Ministry of Health USSR. Moskva. 1980. 97-98.

Sternberg EM. Neural regulation of innate immunity: a coordinated nonspecific host response to pathogens. Nat Rev Immunol. 2006; 6(4): 318-328.

Sydoruk NO, Zukow W. Comparative investigation of immediate effects on neuro-endocrine-immune complex of bioactive water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a. Communication 1. Generic effects. Journal of Education, Health and Sport. 2016; 6(8): 85-101.

Sydoruk NO, Zukow W, Yanchij RI. Integrated quantitative assessment of changes in neuro-endocrine-immune complex and metabolism in rats exposed to acute cold-immobilization stress. Journal of Education, Health and Sport. 2016; 6(9): 724-735.

Thayer JF, Sternberg EM. Neural aspects of immunomodulation: Focus on the vagus nerve. Brain Behav Immun. 2010; 24(8): 1223-1228.

Uchakin PN, Uchakina ON, Tobin BV, Ershov FI. Neuroendocrine immunomodulation [in Russian]. Vestnik Ross AMN. 2007; 9: 26-32.

Vis’tak HI. Relationship between vegetotropic and endocrine, immunotropic as well as clinical effects of bioactive water Naftussya in women with thyroid hyperplasia [in Ukrainian]. Medical Hydrology and Rehabilitation. 2012; 10(2): 37-66.

Вісьтак ГІ, Попович ІЛ. Вегетотропні ефекти біоактивної води Нафтуся та їх ендокринний і імунний супроводи у щурів-самок. Медична гідрологія та реабілітація. 2011; 9(2). 39-57. Vis’tak HI, Popovych IL. Vegetotropic effects of bioactive water Naftussya and their endocrine and immune support in female rats [in Ukrainian]. Medical Hydrology and Rehabilitation. 2011; 9(2): 39-57.

Yushkovs’ka OG. Using information theory to study adaptive responses in the body athletes [in Ukrainian]. Medical Rehabilitation, Kurortology, Physiotherapy. 2001; 1(25): 40-43.



  • There are currently no refbacks.

Copyright (c) 2016 © The Author (s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:

Journal Language(s): English 

PBN Poland



Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2 Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase


Universal Impact Factor 1.78 for year 2012. (

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski