INTEGRATED QUANTITATIVE ASSESSMENT OF CHANGES IN NEURO-ENDOCRINE-IMMUNE COMPLEX AND METABOLISM IN RATS EXPOSED TO ACUTE COLD-IMMOBILIZATION STRESS

Sydoruk O Sydoruk, Walery Zukow, Roman I Yanchij

Abstract


Sydoruk Nadiya O, Zukow Walery, Yanchij Roman I. INTEGRATED QUANTITATIVE ASSESSMENT OF CHANGES IN NEURO-ENDOCRINE-IMMUNE COMPLEX AND METABOLISM IN RATS EXPOSED TO ACUTE COLD-IMMOBILIZATION STRESS. Journal of Education, Health and Sport. 2016;6(9):724-735. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.159218

http://ojs.ukw.edu.pl/index.php/johs/article/view/3915

 

 

 

 

The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).

755 Journal of Education, Health and Sport eISSN 2391-8306 7

© The Author (s) 2016;

This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland

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This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial

use, distribution and reproduction in any medium, provided the work is properly cited.

The authors declare that there is no conflict of interests regarding the publication of this paper.

Received: 02.09.2016. Revised 24.09.2016. Accepted: 24.09.2016.

 

 

 

INTEGRATED QUANTITATIVE ASSESSMENT OF CHANGES IN NEURO-ENDOCRINE-IMMUNE COMPLEX AND METABOLISM IN RATS EXPOSED TO ACUTE COLD-IMMOBILIZATION STRESS

 

Nadiya O Sydoruk1, Walery Zukow2, Roman I Yanchij3

 

1JSC “Dnipro-Beskyd”, Truskavets’, Ukraine dnipro-b@bk.ru

2Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland w.zukow@ukw.edu.pl

3Department of Immunophysiology OO Bogomoletz Institute of Physiology NAS, Kyiv, Ukraine tas@biph.kiev.ua

 

 

Abstracts

Background. It is known that the reaction of the neuroendocrine-immune complex to acute and chronic stress are different. It is also known about sex differences in stress reactions. Previously we have been carry out integrated quantitative estimation of neuroendocrine and immune responses to chronic restraint stress at male rats. The purpose of this study - to carry out integrated quantitative estimation of neuroendocrine, immune and metabolic responses to acute stress at male and female rats. Material and research methods. The experiment is at 58 (28 male and 30 female) white rats Wistar line weighing 170-280 g (Mean=220 g; SD=28 g). The day after acute (water immersion restraint) stress determined HRV, endocrine, immune and metabolic parameters as well as gastric mucosa injuries and comparing them with parameters of intact animals. Results. Acute cold-immobilization stress caused moderate injuries the stomach mucosa as erosions and ulcers. Among the metabolic parameters revealed increased activity Acid Phosphatase, Asparagine and Alanine Aminotranspherase as well as Creatinephosphokinase. It was also found to reduce plasma Testosterone as well as serum Potassium and Phosphate probably due to increased Parathyrine and Mineralocorticoid activity and Sympathotonic shift of sympatho-vagal balance. Integrated quantitative measure manifestations of Acute Stress as mean of modules of Z-Scores makes for 10 metabolic parameters 0,75±0,10 σ and for 8 neuro-endocrine parameters 0,40±0,07 σ. Among immune parameters some proved resistant to acute stress factors, while 10 significant suppressed and 12 activated. Integrated quantitative measure poststressory changes makes 0,73±0,08 σ. Found significant differences integrated status intact males and females, whereas after stress differences are insignificant. Conclusion. The approach to integrated quantitative assessment of neuroendocrine-immune complex and metabolism may be useful for testing the effectiveness streslimiting means.

Keywords: acute stress, HRV, hormones, immunity, metabolism, entropy, male and female rats.


Keywords


acute stress, HRV, hormones, immunity, metabolism, entropy, male and female rats.

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References


REFERENCES

Andreyeva LI, Kozhemyakin LA, Kishkun AA. Modification of the method for determining the lipid peroxide in the test with thiobarbituric acid [in Russian]. Laboratornoye Delo. 1988; 11: 41-43.

Avtandilov GG. Medical Morphometry [in Russian]. Moskva: Meditsina. 1990. 384 p.

Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva: Nauka. 1984. 221 p.

Bazarnova MA. Cytology investigation punctates spleen. In: Guide to practical training in clinical laboratory diagnostics [in Russian]. Kyiv: Vyshcha shkola. 1988. 263-264.

Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.

Chrousos GP. The stress response and immune function: clinical implications. The 1999 Novera H Spector lecture. Neuroimmunomodulation. Perspectives at the new millenium. ANYAS. 2000; 917: 38-67.

Dhabhar FS. Enhancing versus Suppressive Effects of Stress on Immune Function: Implications for Immunoprotection and Immunopathology. Neuroimmunomodulation. 2009; 16(5): 300–317.

Douglas SD, Quie PG. Investigation of Phagocytes in Disease. Churchil. 1981. 110 p.

Dubinina YY, Yefimova LF, Sofronova LN, Geronimus AL. Comparative analysis of the activity of superoxide dismutase and catalase of erythrocytes and whole blood from newborn children with chronic hypoxia [in Russian]. Laboratornoye Delo. 1988; 8: 16-19.

Fajda OI, Drach OV, Barylyak LG, Zukow W. Relationships between Ca/K plasma ratio and parameters of Heart Rate Variability at patients with diathesis urica. Journal of Education, Health and Sport. 2016; 6(1): 295-301.

Garkavi LKh, Kvakina YeB, Kuz’menko TS. Antistress Reactions and Activation Therapy [in Russian]. Мoskva: Imedis, 1998. 654 p.

Gavrilov VB, Mishkorudnaya MI. Spectrophotometric determination of plasma levels of lipid hydroperoxides [in Russian]. Laboratornoye Delo. 1983; 3: 33-36.

Goryachkovskiy AM. Clinical biochemi [in Russian]. Odesa: Astroprint. 1998. 608 p.

Hiller G. Test for the quantitative determination of HDL cholesterol in EDTA plasma with Reflotron ®. Klin Chem. 1987; 33: 895-898.

Hrytsak YaL, Barylyak LG, Zukow W, Popovych IL. Cluster analysis of hormonal constellation at women and men with harmonious and disharmonious general adaptation reactions. Journal of Education, Health and Sport. 2016; 6(4): 141-150.

Instructions for application for recruitment reagents for ELISA investigations hormones in the blood of humans [in Russian]. St. Petersburg: JSC "Alkor Bio". 2000.

Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2): 207-215.

Khaitov RM. Physiology of immune system [in Russian]. Moskva: VINITI RAN. 2005. 428 p.

Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskva: Finansy i Statistika. 1989: 78-138.

Kolyada TI, Volyanskyi YL, Vasilyev NV, Maltsev VI. Adaptation Syndrome and Immunity [in Russian]. Kharkiv: Osnova. 1995. 168 p.

Korolyuk MA, Ivanova MI, Mayorova IG, Tokarev VYe. The method for determining the activity of catalase [in Russian]. Laboratornoye Delo. 1988; 1: 16-19.

Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2015. 349 p.

Lapovets’ LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. Lviv. 2002. 173 p.

Limatibul S, Shore A, Dosch HM, Gelfand EW. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. Clin Exp Immunol. 1978; 33(3): 503-513.

Makarenko YeV. A comprehensive definition of the activity of superoxide dismutase and glutathione reductase in red blood cells in patients with chronic liver disease [in Russian]. Laboratornoye Delo. 1988; 11: 48-50.

Nakamura J, Takada S, Ohtsuka N, Heya T et al. An assessment of gastric ulcers in vivo: enhancement of urinary recovery after oral administration of phenolsulfonphtalein in rats. J Parm Dyn. 1984; 7(7): 485-491.

Nance DM, Sanders VM. Autonomic innervation and regulation of the immune system. Brain Behav Immun. 2007; 21(6): 736-745.

Polovynko IS, Zayats LM, Zukow W, Popovych IL. Neuro-endocrine-immune relationships by chronic stress at male rats. Journal of Health Sciences. 2013; 3(12): 365-374.

Polovynko IS, Zajats LM, Zukow W, Yanchij RI, Popovych IL. Quantitative evaluation of integrated neuro-endocrine and immune responses to chronic stress rats male. Journal of Education, Health and Sport. 2016; 6(8): 154-166.

Popоvych IL. The factor and canonical analysis parameters of neuro-endocrine-immune complex, metabolism and erosive-ulcerose injuries of mucous stomach at rats in conditions of acute water іmmersing stress [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(2): 68-80.

Popоvych IL. Information effects of bioactive water Naftyssya in rats: modulation entropic, prevention desynchronizing and limitation of disharmonizing actions water immersion stress for information components of neuro-endocrine-immune system and metabolism, which correlates with gastroprotective effect [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(3): 50-70.

Popоvych IL. Bioactive water Naftyssya in general like ginseng limits, negates, reverses neuro-hormonal, metabolic and immune pathogens manifestations and strengthens sanogens manifestations acute stress in rats without impacting significantly on performance, not subordinates stress action [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(4): 7-29.

Popovych IL. Stresslimiting Adaptogenic Mechanisms of Biological and Therapeutic Activity of Water Naftussya [in Ukrainian]. Kyiv: Computerpress. 2011. 300 p.

Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-87.

Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18.

Popovych IL, Vis’tak HI, Gumega MD, Ruzhylo SV. Vegetotropic Effects of Bioactive Water Naftussya and their Endocrine-Immune, Metabolic and Hemodynamic Accompaniments [in Ukrainian]. Kyiv: UNESCO-SOCIO. 2014. 163 p.

Schauenstein K, Felsner P, Rinner I, Liebmann PM, Stevenson JR, Westermann J, Haas HS, Cohen RL, Chambers DA. In vivo immunomodulation by peripheral adrenergic and cholinergic agonists/antagonists in rat and mouse models. Neuroimmunomodulation. Perspectives at the new millenium. ANYAS. 2000; 917: 618-627.

Shatalov VN, Polonskiy VM, Bulgakov SA, Vinogradov VA. The ligands of opiate receptors and experimental ulcer. In: Modern methods of diagnosis and treatment of internal diseases [in Russian]. Collection of Scientific Papers IV Main Administration under the Ministry of Health USSR. Moskva. 1980. 97-98.

Sternberg EM. Neural regulation of innate immunity: a coordinated nonspecific host response to pathogens. Nat Rev Immunol. 2006; 6(4): 318-328.

Sydoruk NO, Zukow W. Comparative investigation of immediate effects on neuro-endocrine-immune complex of bioactive water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a. Communication 1. Generic effects. Journal of Education, Health and Sport. 2016; 6(8): 85-101.

Thayer JF, Sternberg EM. Neural aspects of immunomodulation: Focus on the vagus nerve. Brain Behav Immun. 2010; 24(8): 1223-1228.

Uchakin PN, Uchakina ON, Tobin BV, Ershov FI. Neuroendocrine immunomodulation [in Russian]. Vestnik Ross AMN. 2007; 9: 26-32.

Vis’tak HI. Relationship between vegetotropic and endocrine, immunotropic as well as clinical effects of bioactive water Naftussya in women with thyroid hyperplasia [in Ukrainian]. Medical Hydrology and Rehabilitation. 2012; 10(2): 37-66.

Вісьтак Г.І., Попович І.Л. Вегетотропні ефекти біоактивної води Нафтуся та їх ендокринний і імунний супроводи у щурів-самок. Медична гідрологія та реабілітація. 2011.-9,№2.-С. 39-57. Vis’tak HI, Popovych IL. Vegetotropic effects of bioactive water Naftussya and their endocrine and immune support in female rats [in Ukrainian]. Medical Hydrology and Rehabilitation. 2011; 9(2): 39-57.

Yushkovs’ka OG. Using information theory to study adaptive responses in the body athletes [in Ukrainian]. Medical Rehabilitation, Kurortology, Physiotherapy. 2001;1(25): 40-43.




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